Recreating Stable Brachypodium hybridum Allotetraploids by Uniting the Divergent Genomes of B. distachyon and B. stacei.

Autor: Dinh Thi VH; Organization and evolution of complex genomes, Institut National de la Recherche agronomique, Université d'Evry Val d'Essonne, Evry, France., Coriton O; Unité Mixte de Recherches INRA, Agrocampus Rennes-Université Rennes 1, Institut de Génétique, Environnement et Protection des Plantes, Le Rheu, France., Le Clainche I; Organization and evolution of complex genomes, Institut National de la Recherche agronomique, Université d'Evry Val d'Essonne, Evry, France., Arnaud D; Organization and evolution of complex genomes, Institut National de la Recherche agronomique, Université d'Evry Val d'Essonne, Evry, France., Gordon SP; DOE Joint Genome Institute, Walnut Creek, United States of America., Linc G; Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences,Martonvásár, Brunszvik u 2, Hungary., Catalan P; Department of Agriculture and Environmental Sciences, High Polytechnic School of Huesca, University of Zaragoza, Huesca, Spain.; Department of Botany, Institute of Biology, Tomsk State University, Tomsk, Russia., Hasterok R; Department of Plant Anatomy and Cytology, Faculty of Biology and Environmental Protection, University of Silesia,Katowice, Poland., Vogel JP; DOE Joint Genome Institute, Walnut Creek, United States of America.; Department of Plant and Microbial Biology, University of California, Berkeley, United States of America., Jahier J; Unité Mixte de Recherches INRA, Agrocampus Rennes-Université Rennes 1, Institut de Génétique, Environnement et Protection des Plantes, Le Rheu, France., Chalhoub B; Organization and evolution of complex genomes, Institut National de la Recherche agronomique, Université d'Evry Val d'Essonne, Evry, France.; Institute of System and Synthetic Biology, Genopole, Centre National de la Recherche Scientifique, Université d'Evry Val d'Essonne, Université Paris-Saclay, Evry, France.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2016 Dec 09; Vol. 11 (12), pp. e0167171. Date of Electronic Publication: 2016 Dec 09 (Print Publication: 2016).
DOI: 10.1371/journal.pone.0167171
Abstrakt: Brachypodium hybridum (2n = 30) is a natural allopolyploid with highly divergent sub-genomes derived from two extant diploid species, B. distachyon (2n = 10) and B. stacei (2n = 20) that differ in chromosome evolution and number. We created synthetic B. hybridum allotetraploids by hybridizing various lines of B. distachyon and B. stacei. The initial amphihaploid F1 interspecific hybrids were obtained at low frequencies when B. distachyon was used as the maternal parent (0.15% or 0.245% depending on the line used) and were sterile. No hybrids were obtained from reciprocal crosses or when autotetraploids of the parental species were crossed. Colchicine treatment was used to double the genome of the F1 amphihaploid lines leading to allotetraploids. The genome-doubled F1 plants produced a few S1 (first selfed generation) seeds after self-pollination. S1 plants from one parental combination (Bd3-1×Bsta5) were fertile and gave rise to further generations whereas those of another parental combination (Bd21×ABR114) were sterile, illustrating the importance of the parental lineages crossed. The synthetic allotetraploids were stable and resembled the natural B. hybridum at the phenotypic, cytogenetic and genomic levels. The successful creation of synthetic B. hybridum offers the possibility to study changes in genome structure and regulation at the earliest stages of allopolyploid formation in comparison with the parental species and natural B. hybridum.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
Externí odkaz: