The Coexistence of Hypertension and Ovariectomy Additively Increases Cardiac Apoptosis.

Autor: Lin YY; Graduate Institute of Clinical Medical Science, China Medical University, Taichung 40402, Taiwan. charlet8116@gmail.com., Cheng YJ; Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung 40402, Taiwan. s5888116@gmail.com., Hu J; School of Rehabilitation Science, Shanghai University of TCM, Shanghai 201203, China. jasonhwu@126.com., Chu LX; School of Rehabilitation Science, Shanghai University of TCM, Shanghai 201203, China. chulixi2@126.com., Shyu WC; Translational Medicine Research Center, China Medical University Hospital, Taichung 40402, Taiwan. shyu9423@gmail.com.; Graduate Institute of Immunology, China Medical University, Taichung 40402, Taiwan. shyu9423@gmail.com.; Center for Neuropsychiatry, Department of Neurology, China Medical University Hospital, Taichung 40402, Taiwan. shyu9423@gmail.com., Kao CL; Department of Physical Medicine and Rehabilitation, Taipei Veterans General Hospital, Taipei 11217, Taiwan. clkao@vghtpe.gov.tw.; Department of Physical Medicine and Rehabilitation, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan. clkao@vghtpe.gov.tw., Lin TB; Department of Physiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan. tblin2@tmu.edu.tw.; Graduate Institute of Basic Medical Science, College of Medicine, China Medical University, Taichung 40402, Taiwan. tblin2@tmu.edu.tw.; Department of Biotechnology, Asia University, Taichung 41354, Taiwan. tblin2@tmu.edu.tw., Kuo CH; Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung 40402, Taiwan. kuochiahua@gmail.com.; Department of Sports Sciences, University of Taipei, Taipei 11153, Taiwan. kuochiahua@gmail.com., Yang AL; Department of Sports Sciences, University of Taipei, Taipei 11153, Taiwan. yangailun@gmail.com., Lee SD; Graduate Institute of Clinical Medical Science, China Medical University, Taichung 40402, Taiwan. shinda@mail.cmu.edu.tw.; Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung 40402, Taiwan. shinda@mail.cmu.edu.tw.; School of Rehabilitation Science, Shanghai University of TCM, Shanghai 201203, China. shinda@mail.cmu.edu.tw.; Department of Biotechnology, Asia University, Taichung 41354, Taiwan. shinda@mail.cmu.edu.tw.; Department of Occupational Therapy, Asia University, Taichung 41354, Taiwan. shinda@mail.cmu.edu.tw.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2016 Dec 06; Vol. 17 (12). Date of Electronic Publication: 2016 Dec 06.
DOI: 10.3390/ijms17122036
Abstrakt: To investigate whether the coexistence of hypertension and ovariectomy will increase cardiac Fas receptor and mitochondrial-dependent apoptotic pathways, histopathological analysis, the TUNEL assay and Western blotting were performed on the excised hearts from three groups of female spontaneously hypertensive rats (SHR), which were divided into a sham-operated group (SHR-Sham), bilaterally ovariectomized group (SHR-OVX) and normotensive Wistar Kyoto rats (WKY). Compared with the WKY group, the SHR-Sham group exhibited decreased protein levels of ERα, ERβ, p-Akt/Akt, Bcl-2, Bcl-xL and p-Bad and decreased further in the SHR-OVX group, as well as protein levels of t-Bid, Bak, Bad, Bax, cytochrome c , activated caspase-9 and activated caspase-3 (mitochondria-dependent apoptosis) increased in the SHR-Sham group and increased further in the SHR-OVX group. Compared with the WKY group, protein levels of Fas ligand, TNF-α, Fas death receptors, TNFR1, FADD and activated caspase-8 (Fas receptor-dependent apoptosis) increased in the SHR-Sham group, but did not increase in the SHR-OVX group, except Fas ligand and TNF-α. The coexistence of hypertension and ovariectomy attenuated the estrogen receptor survival pathway and appeared to additively increase the cardiac mitochondria-dependent, but not the Fas receptor-dependent apoptosis pathway, which might provide one possible mechanism for the development of cardiac abnormalities in hypertensive postmenopausal women.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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