Enhancing bioactive properties of silk fibroin with diatom particles for bone tissue engineering applications.

Autor:	Le TDH; BIOtech Research Center and European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Department of Industrial Engineering, University of Trento, Trento, Italy., Liaudanskaya V; BIOtech Research Center and European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Department of Industrial Engineering, University of Trento, Trento, Italy.; Department of Biomedical Engineering, Tufts University, Medford, MA, USA., Bonani W; BIOtech Research Center and European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Department of Industrial Engineering, University of Trento, Trento, Italy.; INSTM, Trento Research Unit, Interuniversity Consortium for Science and Technology of Materials, Trento, Italy., Migliaresi C; BIOtech Research Center and European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Department of Industrial Engineering, University of Trento, Trento, Italy.; INSTM, Trento Research Unit, Interuniversity Consortium for Science and Technology of Materials, Trento, Italy., Motta A; BIOtech Research Center and European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Department of Industrial Engineering, University of Trento, Trento, Italy.; INSTM, Trento Research Unit, Interuniversity Consortium for Science and Technology of Materials, Trento, Italy.
Jazyk:	angličtina
Zdroj:	Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2018 Jan; Vol. 12 (1), pp. 89-97. Date of Electronic Publication: 2017 May 19.
DOI:	10.1002/term.2373
Abstrakt:	Many studies have highlighted the role of silicon in human bone formation and maintenance. Silicon, in fact, is considered to nucleate the precipitation of hydroxyapatite and to reduce the bone resorption. For this reason, we have combined silk fibroin (SF) with silicon-releasing diatom particles (DPs), as potential material for bone tissue engineering applications. Sponges of fibroin loaded with different amounts and sizes of DPs were prepared by solvent casting-particulate leaching method, and their morphology, porosity and mechanical properties were evaluated. The biological effect of diatom addition was assessed on human osteosarcoma cell line MG63, a suitable osteoblast-like model, through cell adhesion, metabolic activity and proliferation assays. In addition, alkaline phosphatase activity, osterix and collagen type I production in MG63 cell line were assessed as markers of early bone formation to demonstrate a pro-mineralization potential of scaffolds. Results of the studies showed that addition to fibroin of diatoms particles improved the osteogenic properties of osteoblast-like cells compared with the pure SF. Copyright © 2016 John Wiley & Sons, Ltd. (Copyright © 2016 John Wiley & Sons, Ltd.)
Databáze:	MEDLINE
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