Ovarian and uterine alterations following forced swimming: An immunohistochemical study.

Autor: Seyed Saadat SN; Student Research Center, Guilan University of Medical Sciences, Rasht, Iran., Mohammadghasemi F; Anatomy Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran., Ebrahimi H; Student Research Center, Guilan University of Medical Sciences, Rasht, Iran., Rafati Sajedi H; Student Research Center, Guilan University of Medical Sciences, Rasht, Iran., Chatrnour G; Student Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Jazyk: angličtina
Zdroj: International journal of reproductive biomedicine [Int J Reprod Biomed] 2016 Oct; Vol. 14 (10), pp. 629-636.
Abstrakt: Background: Physical exercise is known to be a stressor stimulus that leads to reproductive disruption.
Objective: The aim of this study was to evaluate the effect of forced swimming on the uterus and ovaries in mice.
Materials and Methods: Adult mice (N=24) were divided into the following three groups: A, control; B, swimming in water (10 o C); and C, swimming in water (23 o C). Swimmers swam for 5 min daily for 5 consecutive days/ wk during 2 wks. An enzyme linked immunosorbent assay was used to determine serum estradiol, follicle stimulating hormone (FSH) and testosterone levels. Immunohistochemistry was performed to study apoptotic cells or estrogen receptor (ER) expression in uterine epithelial cells and ovaries. ANOVA was used for statistical analysis.
Results: Swimming in both groups reduced the serum FSH and estradiol levels (p<0.01) without having a significant effect on the serum testosterone level or percentage of apoptosis in ovarian and uterine tissues (p<0.01) compared with controls. A significant reduction in the number of ERs in the uterus and ovaries, and secondary and graafian follicles were observed in groups B and C compared with controls (p<0.01); however the number of primordial and primary follicles were not significantly changed in the ovaries.
Conclusion: Forced swimming of 2 wks duration reduces the serum levels of FSH and estradiol without having effects on apoptosis in the ovaries or uteri of mice. Over a long period of time, forced swimming may have an adverse effect on fertility.
Competing Interests: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
Databáze: MEDLINE
Externí odkaz: