Acute Fasting Regulates Retrograde Synaptic Enhancement through a 4E-BP-Dependent Mechanism.

Autor:	Kauwe G; Buck Institute for Research on Aging, Novato, CA 94945, USA., Tsurudome K; Buck Institute for Research on Aging, Novato, CA 94945, USA; Department of Physiology, McGill University, Montreal, QC H3G 1Y6, Canada., Penney J; Department of Physiology, McGill University, Montreal, QC H3G 1Y6, Canada., Mori M; Buck Institute for Research on Aging, Novato, CA 94945, USA., Gray L; Buck Institute for Research on Aging, Novato, CA 94945, USA., Calderon MR; Buck Institute for Research on Aging, Novato, CA 94945, USA., Elazouzzi F; Department of Physiology, McGill University, Montreal, QC H3G 1Y6, Canada., Chicoine N; Buck Institute for Research on Aging, Novato, CA 94945, USA., Sonenberg N; Department of Biochemistry and Cancer Center, McGill University, Montreal, QC H3G 1Y6, Canada., Haghighi AP; Buck Institute for Research on Aging, Novato, CA 94945, USA; Department of Physiology, McGill University, Montreal, QC H3G 1Y6, Canada. Electronic address: phaghighi@buckinstitute.org.
Jazyk:	angličtina
Zdroj:	Neuron [Neuron] 2016 Dec 21; Vol. 92 (6), pp. 1204-1212. Date of Electronic Publication: 2016 Dec 01.
DOI:	10.1016/j.neuron.2016.10.063
Abstrakt:	While beneficial effects of fasting on organismal function and health are well appreciated, we know little about the molecular details of how fasting influences synaptic function and plasticity. Our genetic and electrophysiological experiments demonstrate that acute fasting blocks retrograde synaptic enhancement that is normally triggered as a result of reduction in postsynaptic receptor function at the Drosophila larval neuromuscular junction (NMJ). This negative regulation critically depends on transcriptional enhancement of eukaryotic initiation factor 4E binding protein (4E-BP) under the control of the transcription factor Forkhead box O (Foxo). Furthermore, our findings indicate that postsynaptic 4E-BP exerts a constitutive negative input, which is counteracted by a positive regulatory input from the Target of Rapamycin (TOR). This combinatorial retrograde signaling plays a key role in regulating synaptic strength. Our results provide a mechanistic insight into how cellular stress and nutritional scarcity could acutely influence synaptic homeostasis and functional stability in neural circuits. (Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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