Identification and interspecies transmission of a novel bocaparvovirus among different bat species in China.

Autor:	Lau SKP; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong SAR, PR China.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong SAR, PR China.; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR, PR China., Ahmed SS; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China., Yeung HC; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China., Li KSM; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China., Fan RYY; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China., Cheng TYC; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China., Cai JP; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China., Wang M; Guangzhou Center for Disease Control and Prevention, Guangzhou, PR China., Zheng BJ; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong SAR, PR China.; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong SAR, PR China.; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China., Wong SSY; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong SAR, PR China.; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR, PR China.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong SAR, PR China., Woo PCY; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong SAR, PR China.; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China.; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong SAR, PR China., Yuen KY; Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China.; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong SAR, PR China.; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR, PR China.; State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong SAR, PR China.
Jazyk:	angličtina
Zdroj:	The Journal of general virology [J Gen Virol] 2016 Dec; Vol. 97 (12), pp. 3345-3358. Date of Electronic Publication: 2016 Oct 25.
DOI:	10.1099/jgv.0.000645
Abstrakt:	We report the discovery of a novel bocaparvovirus, bat bocaparvovirus (BtBoV), in one spleen, four respiratory and 61 alimentary samples from bats of six different species belonging to three families, Hipposideridae, Rhinolophidae and Vespertilionidae. BtBoV showed a higher detection rate in alimentary samples of Rhinolophus sinicus (5.7 %) than those of other bat species (0.43-1.59 %), supporting R. sinicus as the primary reservoir and virus spillover to accidental bat species. BtBoV peaked during the lactating season of R. sinicus, and it was more frequently detected among female than male adult bats (P<0.05), and among lactating than non-lactating female bats (P<0.0001). Positive BtBoV detection was associated with lower body weight in lactating bats (P<0.05). Ten nearly complete BtBoV genomes from three bat species revealed a unique large ORF1 spanning NS1 and NP1 in eight genomes and conserved splicing signals leading to multiple proteins, as well as a unique substitution in the conserved replication initiator motif within NS1. BtBoV was phylogenetically distantly related to known bocaparvoviruses with ≤57.3 % genome identities, supporting BtBoV as a novel species. Ms-BtBoV from Miniopterus schreibersii and Hp-BtBoV from Hipposideros pomona demonstrated 97.2-99.9 % genome identities with Rs-BtBoVs from R. sinicus, supporting infection of different bat species by a single BtBoV species. Rs-BtBoV_str15 represents the first bat parvovirus genome with non-coding regions sequenced, which suggested the presence of head-to-tail genomic concatamers or episomal forms of the genome. This study represents the first to describe interspecies transmission in BoVs. The high detection rates in lactating female and juvenile bats suggest possible vertical transmission of BtBoV.
Databáze:	MEDLINE
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