[Evaluation of an immunochromatographic test for the detection of OXA-48 carbapenemase].

Autor:	Mediavilla-Gradolph C, Sáinz-Rodriguez R, Valverde-Troya M, de Toro-Peinado I, Bermudez-Ruíz MP, Palop-Borrás B; Begoña Palop-Borrás, Laboratorio de Microbiología, Hospital Regional Universitario de Málaga, Avda. Carlos Haya, s/n 29010 Málaga, Spain. bpalop@hotmail.com.
Jazyk:	Spanish; Castilian
Zdroj:	Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia [Rev Esp Quimioter] 2017 Feb; Vol. 30 (1), pp. 45-49. Date of Electronic Publication: 2016 Nov 25.
Abstrakt:	Objective: Detection and differentiation of various types of carbapenemases is crucial to their control and dissemination. OXA-48 is the most common carbapenemase in Spain and in our environment. The aim of this study is the evaluation of a new immunochromatographic test OXA-48 Card letitest (Coris, BioConcept Belgium) to detect this carbapenemase from solid media. Methods: During the last year 151 strains of carbapenemase producing bacteria have been isolated, of which 136 were OXA-48 (126 Klebsiella pneumoniae, 1 Klebsiella oxytoca, 5 Escherichia coli, 4 Enterobacter cloacae), and 15 producing other carbapenemases . These 15 strains with other 73 carrying other resistance mechanisms (54 extended-spectrum β-lactamases producers and 19 with other mechanisms) were used as negative controls. Results: One hundred and thirty six strains carrying OXA-48 were positive with the test OXA-48 Card letitest and the 88 species used as controls were negative, resulting in a sensitivity and specificity of 100%. Conclusions: The OXA-48 Card letitest is simple, quick, safe and cheap (approx. 6€/test) and can be used in microbiology laboratories to confirm the production of OXA-48 carbapenemase in clinical isolates.
Databáze:	MEDLINE
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