| Autor: |
Carvalho ND; Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brazil., Mendonça RZ; Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brazil., Oliveira MI; Núcleo de Doenças Respiratórias, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, São Paulo, CEP 01246-902, Brazil., Curti SP; Núcleo de Doenças Respiratórias, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, São Paulo, CEP 01246-902, Brazil., Barbosa TF; Núcleo de Doenças Respiratórias, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, São Paulo, CEP 01246-902, Brazil., Silva PE; Núcleo de Doenças Respiratórias, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, São Paulo, CEP 01246-902, Brazil., Taniwaki NN; Núcleo de Microcopia Eletrônica, Instituto Adolfo Lutz, São Paulo, Brazil., Tonelotto M; Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brazil., Giovanni DN; Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brazil., Moraes RH; Laboratório Especial de Coleções Zoológicas, Instituto Butantan, São Paulo, Brazil., Figueiredo CA; Núcleo de Doenças Respiratórias, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 355, São Paulo, CEP 01246-902, Brazil. figueiredocris@uol.com.br. |
| Abstrakt: |
Many active principles produced by animals, plants and microorganisms have been employed in the development of new drugs for the treatment of human diseases. Among animals known to produce pharmacologically active molecules that interfere in human cell physiology. Rubella virus (genus Rubivirus, family Togaviridae) is a single stranded RNA virus of positive genome polarity. Rubella virus infection of susceptible women during the first trimester of pregnancy often results in long-term virus persistence in the fetus causing multiple organ abnormalities. Potent antiviral activity against rubella virus (RV) has been observed in the hemolymph of Podalia sp. (Lepidoptera: Megalopygidae). This study evaluated the effect of hemolymph on RV infected Statens Serum Institute Rabbit Cornea (SIRC) cells. Results of cell viability and cell proliferation assays indicated that hemolymph was not toxic to cultured SIRC cells. Viral binding assay, antiviral assay, PCR, real-time PCR, and transmission electron microscopy were used to demonstrate that hemolymph in post-treatment could inhibit the production of infectious RV particles. Specifically, hemolymph was found to inhibit RV adsorption to the SIRC cells. |
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