Microevolutionary analyses of Pythium insidiosum isolates of Brazil and Thailand based on exo-1,3-β-glucanase gene.

Autor: Ribeiro TC; Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Programa de Pós-graduação em Farmacologia (PPGF), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., Weiblen C; Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Programa de Pós-graduação em Medicina Veterinária (PPGMV), UFSM, Santa Maria, RS, Brazil., de Azevedo MI; Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Programa de Pós-graduação em Farmacologia (PPGF), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., de Avila Botton S; Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Programa de Pós-graduação em Medicina Veterinária (PPGMV), UFSM, Santa Maria, RS, Brazil., Robe LJ; Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Pereira DI; Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil., Monteiro DU; Laboratório de Parasitologia Humana e Biologia Molecular, UFSM, Santa Maria, RS, Brazil., Lorensetti DM; Laboratório de Parasitologia Humana e Biologia Molecular, UFSM, Santa Maria, RS, Brazil., Santurio JM; Laboratório de Pesquisas Micológicas, Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Programa de Pós-graduação em Farmacologia (PPGF), Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: janio.santurio@gmail.com.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2017 Mar; Vol. 48, pp. 58-63. Date of Electronic Publication: 2016 Nov 25.
DOI: 10.1016/j.meegid.2016.11.020
Abstrakt: Pythium insidiosum is an important oomycete due to its ability to infect humans and animals. It causes pythiosis, a disease of difficult treatment that occurs more frequently in humans in Thailand and in horses in Brazil. Since cell-wall components are frequently related to host shifts, we decided here to use sequences from the exo-1,3-β-glucanase gene (exo1), which encodes an immunodominant protein putatively involved in cell wall remodeling, to investigate the microevolutionary relationships of Brazilian and Thai isolates of P. insidiosum. After neutrality ratification, the phylogenetic analyses performed through Maximum parsimony (MP), Neighbor-joining (NJ), Maximum likelihood (ML), and Bayesian analysis (BA) strongly supported Thai isolates being paraphyletic in relation to those from Brazil. The structure recovered by these analyses, as well as by Spatial Analysis of Molecular Variance (SAMOVA), suggests the subdivision of P. insidiosum into three clades or population groups, which are able to explain almost 81% of the variation encountered for exo1. Moreover, the two identified Thai clades were almost as strongly differentiated between each other, as they were from the Brazilian clade, suggesting an ancient Asian subdivision. The derived positioning in the phylogenetic tree, linked to the lower diversity values and the recent expansion signs detected for the Brazilian clade, further support this clade as derived in relation to the Asian populations. Thus, although some patterns presented here are compatible with those recovered with different molecular markers, exo1 was revealed to be a good marker for studying evolution in Pythium, providing robust and strongly supported results with regard to the patterns of origin and diversification of P. insidiosum.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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