DNA damage induced by coal dust, fly and bottom ash from coal combustion evaluated using the micronucleus test and comet assay in vitro.

Autor: Matzenbacher CA; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil., Garcia AL; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil., Dos Santos MS; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil., Nicolau CC; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil., Premoli S; Research Center Product and Development (CEPPED), Postgraduate Program in Genetics and Applied Toxicology (PPGGTA), Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil., Corrêa DS; Research Center Product and Development (CEPPED), Postgraduate Program in Genetics and Applied Toxicology (PPGGTA), Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil., de Souza CT; Ion Implantation Laboratory, Institute of Physics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil., Niekraszewicz L; Ion Implantation Laboratory, Institute of Physics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil., Dias JF; Ion Implantation Laboratory, Institute of Physics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil., Delgado TV; Coal Analysis and Rocks Oil Generators Laboratory, Institute of Geosciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil., Kalkreuth W; Coal Analysis and Rocks Oil Generators Laboratory, Institute of Geosciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil., Grivicich I; Laboratory of Cancer Biology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil., da Silva J; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil. Electronic address: juliana.silva@ulbra.br.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2017 Feb 15; Vol. 324 (Pt B), pp. 781-788. Date of Electronic Publication: 2016 Nov 22.
DOI: 10.1016/j.jhazmat.2016.11.062
Abstrakt: Coal mining and combustion generating huge amounts of bottom and fly ash are major causes of environmental pollution and health hazards due to the release of polycyclic aromatic hydrocarbons (PAH) and heavy metals. The Candiota coalfield in Rio Grande do Sul, is one of the largest open-cast coal mines in Brazil. The aim of this study was to evaluate genotoxic and mutagenic effects of coal, bottom ash and fly ash samples from Candiota with the comet assay (alkaline and modified version) and micronucleus test using the lung fibroblast cell line (V79). Qualitative and quantitative analysis of PAH and inorganic elements was carried out by High Performance Liquid Chromatography (HPLC) and by Particle-Induced X-ray Emission (PIXE) techniques respectively. The samples demonstrated genotoxic and mutagenic effects. The comet assay modified using DNA-glicosilase formamidopirimidina (FPG) endonuclease showed damage related to oxidative stress mechanisms. The amount of PAHs was higher in fly ash followed by pulverized coal. The amount of inorganic elements was highest in fly ash, followed by bottom ash. It is concluded that the samples induce DNA damage by mechanisms that include oxidative stress, due to their complex composition, and that protective measures have to be taken regarding occupational and environmental hazards.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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