Salinomycin reduces stemness and induces apoptosis on human ovarian cancer stem cell.

Autor:	Lee HG; Institute for Cancer Research, Keimyung University, School of Medicine, Daegu, Korea., Shin SJ; Department of Obstetrics and Gynecology, Keimyung University, School of Medicine, Daegu, Korea., Chung HW; Department of Obstetrics and Gynecology, Keimyung University, School of Medicine, Daegu, Korea., Kwon SH; Department of Obstetrics and Gynecology, Keimyung University, School of Medicine, Daegu, Korea., Cha SD; Department of Obstetrics and Gynecology, Keimyung University, School of Medicine, Daegu, Korea., Lee JE; Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA., Cho CH; Institute for Cancer Research, Keimyung University, School of Medicine, Daegu, Korea.; Department of Obstetrics and Gynecology, Keimyung University, School of Medicine, Daegu, Korea. chcho@kmu.ac.kr.
Jazyk:	angličtina
Zdroj:	Journal of gynecologic oncology [J Gynecol Oncol] 2017 Mar; Vol. 28 (2), pp. e14. Date of Electronic Publication: 2016 Nov 14.
DOI:	10.3802/jgo.2017.28.e14
Abstrakt:	Objective: Cancer stem cells (CSCs) represent a subpopulation of undifferentiated tumorigenic cells thought to be responsible for tumor initiation, maintenance, drug resistance, and metastasis. The role of CSCs in drug resistance and relapse of cancers could significantly affect outcomes of ovarian cancer patient. Therefore, therapies that target CSCs could be a promising approach for ovarian cancer treatment. The antibiotic salinomycin has recently been shown to deplete CSCs. In this study, we evaluated the effect of salinomycin on ovarian cancer stem cells (OCSCs), both alone and in combination with paclitaxel (PTX). Methods: The CD44⁺CD117⁺CSCs were obtained from the ascitic fluid of patients with epithelial ovarian cancer by using an immune magnetic-activated cell sorting system. OCSCs were treated with PTX and salinomycin either singly or in combination. Cell viability and apoptosis assays were performed and spheroid-forming ability was measured. The expression of sex determining region Y-box 2 (SOX2) and octamer-binding transcription factor 3/4 (OCT3/4) mRNA was determined using reverse transcription polymerase chain reaction, and protein expression was observed using western blot analysis. Results: Treatment with salinomycin alone reduced the stemness marker expression and spheroid-forming ability of OCSCs. Treatment with PTX alone did not decrease the viability of OCSCs. Treatment with a combination of salinomycin decreased the viability of OCSCs and promoted cell apoptosis. The enhancement of combination treatment was achieved through the apoptosis as determined by annexin V/propidium iodide (PI) staining, caspase-3 activity, and DNA fragmentation assay. Conclusion: Based on our findings, combining salinomycin with other anti-cancer therapeutic agents holds promise as an ovarian cancer treatment approach that can target OCSCs. Competing Interests: No potential conflict of interest relevant to this article was reported. (Copyright © 2017. Asian Society of Gynecologic Oncology, Korean Society of Gynecologic Oncology)
Databáze:	MEDLINE
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