Phytochemical screening and analgesic profile of the lyophilized aqueous extract obtained from Chrysobalanus icaco leaves in experimental protocols.

Autor: Araújo-Filho HG; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., Dias JD; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., Quintans-Júnior LJ; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., Santos MR; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., White PA; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., Barreto RS; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., Barreto AS; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., Estevam CS; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., Araujo SS; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil., Almeida JR; b Department of Pharmaceutical Sciences , Federal University of Vale do São Francisco , Petrolina , PE , Brazil., Menezes IR; c Department of Biological Chemistry , Regional University of Cariri , Crato , CE , Brazil., Coutinho HD; c Department of Biological Chemistry , Regional University of Cariri , Crato , CE , Brazil., Quintans JS; a Department of Physiology , Federal University of Sergipe , São Cristóvão , SE , Brazil.
Jazyk: angličtina
Zdroj: Pharmaceutical biology [Pharm Biol] 2016 Dec; Vol. 54 (12), pp. 3055-3062.
DOI: 10.1080/13880209.2016.1204618
Abstrakt: Context: Chrysobalanus icaco L. (Chrysobalanaceae) has been used for the treatment of abdominal pain and cramps.
Objective: Assess the chemical and pharmacological profile of the lyophilized aqueous extract from C. icaco leaves (AEC).
Materials and Methods: Chromatographic methods were used to assess compounds from AEC. Mice were treated with vehicle (control group) or AEC (100, 200 or 400 mg/kg, p.o.) (group with 7-8 mice) and the analgesic profile was assessed employing the acetic acid-induced writhing, formalin, hot plate tests and hyperalgesia induced by carrageenan (CG) or tumour necrosis factor-alpha. The animal motor performance was assessed using rota-rod and grip strength tests.
Results: The chromatographic profile of AEC demonstrated the presence of terpenoid compounds. The acute pretreatment with AEC, at all doses, produced a significant (p < 0.01) inhibition of painful bahaviour (11.4 ± 3.6; 10.3 ± 2.8; 11.3 ± 2.2) when compared to the control group (24.7 ± 4.7) in acetic acid-induced writhing test. In the formalin test, AEC were effective in the second phase (p < 0.01) (57.2 ± 10.3; 56.3 ± 9.2; 54.7 ± 8.9) when compared to control group (121.9 ± 18.5). No response was observed in the hot plate test. The higher dose of AEC produced a significant (p < 0.01 or p < 0.05) inhibitory effect on the mechanical hyperalgesia test. AEC did not affect the motor performance of the mice.
Discussion: The terpenoids from AEC are known for its analgesic and anti-inflammatory properties. So, these results corroborate the experiments using the AEC in inflammatory pain protocols.
Conclusion: Our results suggest that AEC act against inflammatory pain.
Databáze: MEDLINE
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