nor-BNI Antagonism of Kappa Opioid Agonist-Induced Reinstatement of Ethanol-Seeking Behavior.

Autor: Harshberger E; Department of Psychology, Grand Valley State University, Allendale, MI, USA., Gilson EA; Department of Psychology, Grand Valley State University, Allendale, MI, USA., Gillett K; Department of Psychology, Grand Valley State University, Allendale, MI, USA., Stone JH; Department of Psychology, Grand Valley State University, Allendale, MI, USA., El Amrani L; Department of Psychology, Grand Valley State University, Allendale, MI, USA., Valdez GR; Department of Psychology, Grand Valley State University, Allendale, MI, USA.
Jazyk: angličtina
Zdroj: Journal of addiction [J Addict] 2016; Vol. 2016, pp. 1084235. Date of Electronic Publication: 2016 Nov 06.
DOI: 10.1155/2016/1084235
Abstrakt: Recent work suggests that the dynorphin (DYN)/kappa opioid receptor (KOR) system may be a key mediator in the behavioral effects of alcohol. The objective of the present study was to examine the ability of the KOR antagonist norbinaltorphimine (nor-BNI) to attenuate relapse to ethanol seeking due to priming injections of the KOR agonist U50,488 at time points consistent with KOR selectivity. Male Wistar rats were trained to self-administer a 10% ethanol solution, and then responding was extinguished. Following extinction, rats were injected with U50,488 (0.1-10 mg/kg, i.p.) or saline and were tested for the reinstatement of ethanol seeking. Next, the ability of the nonselective opioid receptor antagonist naltrexone (0 or 3.0 mg/kg, s.c.) and nor-BNI (0 or 20.0 mg/kg, i.p.) to block U50,488-induced reinstatement was examined. Priming injections U50,488 reinstated responding on the previously ethanol-associated lever. Pretreatment with naltrexone reduced the reinstatement of ethanol-seeking behavior. nor-BNI also attenuated KOR agonist-induced reinstatement, but to a lesser extent than naltrexone, when injected 24 hours prior to injections of U50,488, a time point that is consistent with KOR selectivity. While these results suggest that activation of KORs is a key mechanism in the regulation of ethanol-seeking behavior, U50,488-induced reinstatement may not be fully selective for KORs.
Competing Interests: The authors declare that they have no competing interests.
Databáze: MEDLINE
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