| Autor: |
Song XR; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China., Tang SH; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China., Tang ZQ; Shanghai Tobacco Co., Ltd, Shanghai, China., Yang XM; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China., Wang XW; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China., Wang XF; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China., Xu PJ; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China xupengjun@163.com., Ren GW; Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China xupengjun@163.com. |
| Abstrakt: |
We previously described a novel densovirus [Myzus persicae nicotianae densovirus (MpnDV)] infecting M. persicae nicotianae (Hemiptera: Aphididae) with 34% prevalence. This single-stranded DNA virus has a 5480-nucleotide ambisense genome and belongs to the Densovirinae subfamily within the family Parvoviridae. In the present study, we estimated the genetic diversity of MpnDV using partial nonstructural protein (NS) and capsid protein (VP) gene sequences from 10 locations in China. First, we identified MpnDV-positive samples by amplifying a 445-bp fragment with primers MpDVF/MpDVR. Subsequently, we amplified and sequenced COI genes with primers MpCOIF/ MpCOIR, and partial NS and VP sequences with primers MpnDVF1/MpnDVR1. The respective 655-, 1461-, and 423-bp COI, NS, and VP fragments were used to analyze the genetic diversity of MpnDV using MEGA 6.0 and DnaSP 5.0. The high level of identity shared by all COI sequences (>99%) suggested that the aphids sampled were of the same species, and indicated population homogeneity across the 10 locations investigated. The nucleotide diversity of MpnDV sequences (0.0020 ± 0.0025) was significantly higher than that of the COI genes (0.0002 ± 0.0005). The pairwise fixation index for MpnDV was 0.832, and the total gene flow was 0.05. Phylogenetic analysis revealed that the MpnDV haplotypes clustered according to geographical location, except for those from the Liaoning and Shanxi provinces. In conclusion, MpnDV demonstrated a low level of gene flow and high genetic diversity, suggesting that it is vertically transmitted, and implying that endosymbiotic viruses could be used as markers in studies of insect population genetics. |
