Cat's whiskers (Orthosiphon stamineus) tea modulates arthritis pathogenesis via the angiogenesis and inflammatory cascade.

Autor: Tabana YM; Faculty of pharmacy, University Technology Mara (UiTM), Bertam campus, Penang, Malaysia. Yasser.tabana@Hotmail.com., Al-Suede FS; EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Pulau Pinang, Malaysia., Ahamed MB; EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Pulau Pinang, Malaysia., Dahham SS; EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Pulau Pinang, Malaysia., Hassan LE; EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Pulau Pinang, Malaysia., Khalilpour S; Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, Italy., Taleb-Agha M; EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Pulau Pinang, Malaysia., Sandai D; Advanced Medical and Dental Institute (IPPT), Universiti Sains Malaysia, Bertam, 13200, Kepala Batas, Penang, Malaysia., Majid AS; Department of Pharmacology, Quest International University, Perak, Malaysia., Majid AM; EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Pulau Pinang, Malaysia. aminmalikshah@gmail.com.
Jazyk: angličtina
Zdroj: BMC complementary and alternative medicine [BMC Complement Altern Med] 2016 Nov 24; Vol. 16 (1), pp. 480. Date of Electronic Publication: 2016 Nov 24.
DOI: 10.1186/s12906-016-1467-4
Abstrakt: Background: Orthosiphon stamineus is used traditionally to treat gout, arthritis, and inflammatory related conditions. The in vitro anti-inflammatory effects of the plant have been scientifically investigated. The goal of the present study was to evaluate the potential of the 50% ethanol extract of O. stamineus (EOS) to treat rheumatoid arthritis.
Methods: Anti-arthritic activity was assessed using the in vitro heat denaturation test and the (FCA)-induced arthritis model. Efficacy was assessed by measurements of paw edema and granulation, X-ray radiography, fluorescence molecular tomography (FMT), and histological evaluation. Levels of (TNF-α), interleukin-1 (IL-1), and (COX-1 and COX-2) were analyzed in vitro in lipopolysaccharide (LPS)-stimulated human macrophage (U937). TNF-α and IL-1 levels in the serum samples of arthritic rats were also measured using an ELISA kit.
Results: Treatment with EOS resulted in dose-dependent inhibition of paw edema in acute and chronic models of inflammation. It also inhibited significantly the production of TNF-α, IL-1 COX-1, and COX-2 in the LPS-stimulated U937 macrophages. EOS significantly suppressed FCA-induced paw edema as well as the serum levels of TNF-α and IL-1. X-rays of the synovial joint of the hind leg showed considerable improvement in joint integrity and recovery of tibia-talus bones from degeneration and osteoporotic lesions. Histology of proximal interphalangeal joints of EOS-treated animals showed obvious protection of cartilage and soft tissue. Finally, FMT analysis strongly supported the anti-arthritic effect of EOS. EOS had high phenolic and total flavonoid content as well as strong antioxidant activity.
Conclusions: Results illustrated that the anti-arthritic properties of O. stamineus could be beneficial for prevention and management of rheumatoid arthritis and other chronic inflammatory disorders. Illustration of the Anti- arthritis efficacy of Orthosiphon Stamineus standardized extract.
Databáze: MEDLINE
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