Immunohistochemical comparative analysis of lymphatic vessel density and VEGF-C expression in squamous cell carcinomas of the tongue between young and old patients.

Autor: Marinho Bezerra de Oliveira Moura J; Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil. Electronic address: jamilemarinho@hotmail.com., de Souza Martins Câmara AC; Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Weege Nonaka CF; Postgraduate Program in Dentistry, State University of Paraíba, Campina Grande, PB, Brazil., Pinto LP; Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil., de Souza LB; Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Jazyk: angličtina
Zdroj: Pathology, research and practice [Pathol Res Pract] 2016 Dec; Vol. 212 (12), pp. 1095-1101. Date of Electronic Publication: 2016 Nov 02.
DOI: 10.1016/j.prp.2016.10.015
Abstrakt: Background: Squamous cell carcinoma (SCC) of the oral cavity is a malignant epithelial tumor that most commonly involves the tongue, and mainly affects men between the fifth and seventh decades of life. This study compared the lymphatic vessel density and VEGF-C expression in SCCs of the tongue between young and old patients.
Methods: Thirty-four SCCs of the tongue, 17 diagnosed in young patients (≤40y) and 17 diagnosed in old patients (>50y), were evaluated by immunohistochemistry. Lymphatic microdensity was determined based on the number of vessels immunoreactive to anti-D2-40 antibody. The percentage of neoplastic cells exhibiting cytoplasmic staining for VEGF-C was established for each case.
Results: Assessment of intratumoral lymphatic microdensity (ILMD) disclosed a median number of 6.20 lymphatic vessels in young patients and of 6.60 in old patients (p=0.809). Analysis of peritumoral lymphatic microdensity (PLMD) revealed a median number of 3.60 lymphatic vessels in young patients and of 3.40 in old patients (p=0.769). In the tumor core, analysis of the percentage of immunopositive cells for VEGF-C revealed a median number of 95.95% in young patients and of 55.48% in old patients (p<0.001). In the deep invasive front, the median number of immunopositive cells for VEGF-C was 97.93% in young patients and 66.09% in old patients (p<0.001).
Conclusions: The results of this study suggest that the more aggressive biological behavior of SCC of the tongue in young patients may be related to a higher expression of VEGF-C.
(Copyright © 2016 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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