Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes.

Autor: Kim K; Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea., Lee S; Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea., Yoon J; Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea., Heo J; Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea., Choi C; Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea., Park Y; Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.; TOMOCUBE, Inc., Daejeon 34051, Republic of Korea.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2016 Nov 22; Vol. 6, pp. 36815. Date of Electronic Publication: 2016 Nov 22.
DOI: 10.1038/srep36815
Abstrakt: Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, have limited ability to provide quantitative information on individual LDs and have limited capability for three-dimensional (3-D) imaging of LDs in live cells especially for fast acquisition of 3-D dynamics. Here, we present an optical method based on 3-D quantitative phase imaging to measure the 3-D structural distribution and biochemical parameters (concentration and dry mass) of individual LDs in live cells without using exogenous labelling agents. The biochemical change of LDs under oleic acid treatment was quantitatively investigated, and 4-D tracking of the fast dynamics of LDs revealed the intracellular transport of LDs in live cells.
Competing Interests: Prof. Park has financial interest in Tomocube Inc., a company that commercializes optical diffraction tomography instruments and phase imaging instruments and is one of the sponsors of the work.
Databáze: MEDLINE
Externí odkaz: