Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains Using High Throughput Sequencing.

Autor:	Machado MP; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal., Ribeiro-Gonçalves B; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal., Silva M; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal., Ramirez M; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal., Carriço JA; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal. jcarrico@fm.ul.pt.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1520, pp. 331-356.
DOI:	10.1007/978-1-4939-6634-9_20
Abstrakt:	High-Throughput Sequencing (HTS) technologies transformed the microbial typing and molecular epidemiology field by providing the cost-effective ability for researchers to probe draft genomes, not only for epidemiological markers but also for antibiotic resistance and virulence determinants. In this chapter, we provide protocols for the analysis of HTS data for the determination of multilocus sequence typing (MLST) information and for determining presence or absence of antibiotic resistance genes.
Databáze:	MEDLINE
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