Gastroprotective and ulcer healing effects of hydroethanolic extract of leaves of Caryocar coriaceum: Mechanisms involved in the gastroprotective activity.

Autor: de Lacerda Neto LJ; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil., Ramos AG; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil., Santos Sales V; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil., de Souza SD; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil., Dos Santos AT; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil., de Oliveira LR; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil., Kerntopf MR; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil., de Albuquerque TR; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil., Coutinho HD; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil. Electronic address: hdmcoutinho@gmail.com., Quintans-Júnior LJ; Department of Physiology, Federal University of Sergipe, Campus Universitário Prof. Aloísio de Campos, S/N São Cristovão, 49100001, Aracaju, SE, Brazil., Wanderley AG; Department of Physiology and Pharmacology, Federal University of Pernambuco - UFPE, Av. Prof. Moraes Rego, S/N, Recife, PE, Brazil., de Menezes IR; Chemical-Biology Department, Regional University of Cariri - URCA, Cel. Antonio Luis 1161, Crato, CE, Brazil. Electronic address: Irwin.alencar@urca.br.
Jazyk: angličtina
Zdroj: Chemico-biological interactions [Chem Biol Interact] 2017 Jan 05; Vol. 261, pp. 56-62. Date of Electronic Publication: 2016 Nov 18.
DOI: 10.1016/j.cbi.2016.11.020
Abstrakt: This work aimed to determine the chemical fingerprint of hydroethanolic extract of leaves of Caryocar coriaceum (HELCC) and the gastroprotective activity. The chemical fingerprint of HELCC was analyzed by HPLC-DAD, to quantify total phenols and flavonoids were carried out by Folin-Ciocalteu reagent and aluminum chloride assay, while in vitro antioxidant activity was evaluated by the DPPH method. The methods used to determine pharmacological activity were: gastroprotective screening test in classical models of induced acute and chronic gastric lesions and physical barrier test. Further assays were performed to demonstrate the involvement of NO, prostaglandins, ATP-dependent potassium channels, TRPV, noradrenergic α2 receptors, histamines, and opioids. The DPPH method demonstrated the antioxidant activity of the extract, in vitro, explained by the presence of polyphenols and flavonoids. Oral administration of the extract, previously dissolved in deionized water, at a dose of 100 mg/kg decreased the lesions induced by indomethacin, acidified ethanol, ethanol and acetic acid by 75.0, 72.8, 69.4 and 86.2% respectively. It was demonstrated that opioid receptors, α 2 -adrenergic receptors and primary afferent neurons sensitive to capsaicin were involved in the mechanism of gastric protection, in addition to the contribution of NO and prostaglandins. The results show that extract is a promising candidate for the treatment of gastric ulcers.
(Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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