Integrated proteomics and metabolomics suggests symbiotic metabolism and multimodal regulation in a fungal-endobacterial system.

Autor: Li Z; Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.; Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Yao Q; Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Dearth SP; Department of Chemistry, University of Tennessee, Knoxville, TN, USA., Entler MR; Graduate School of Genome Science and Technology, University of Tennessee-Oak Ridge National Laboratory, Knoxville, TN, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA., Castro Gonzalez HF; Department of Chemistry, University of Tennessee, Knoxville, TN, USA., Uehling JK; Department of Biology, Duke University, Durham, NC, USA., Vilgalys RJ; Department of Biology, Duke University, Durham, NC, USA., Hurst GB; Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Campagna SR; Department of Chemistry, University of Tennessee, Knoxville, TN, USA., Labbé JL; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA., Pan C; Computer Science and Mathematics Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA.; Graduate School of Genome Science and Technology, University of Tennessee-Oak Ridge National Laboratory, Knoxville, TN, USA.; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, USA.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2017 Mar; Vol. 19 (3), pp. 1041-1053. Date of Electronic Publication: 2017 Jan 30.
DOI: 10.1111/1462-2920.13605
Abstrakt: Many plant-associated fungi host endosymbiotic endobacteria with reduced genomes. While endobacteria play important roles in these tri-partite plant-fungal-endobacterial systems, the active physiology of fungal endobacteria has not been characterized extensively by systems biology approaches. Here, we use integrated proteomics and metabolomics to characterize the relationship between the endobacterium Mycoavidus sp. and the root-associated fungus Mortierella elongata. In nitrogen-poor media, M. elongata had decreased growth but hosted a large and growing endobacterial population. The active endobacterium likely extracted malate from the fungal host as the primary carbon substrate for energy production and biosynthesis of phospho-sugars, nucleobases, peptidoglycan and some amino acids. The endobacterium obtained nitrogen by importing a variety of nitrogen-containing compounds. Further, nitrogen limitation significantly perturbed the carbon and nitrogen flows in the fungal metabolic network. M. elongata regulated many pathways by concordant changes on enzyme abundances, post-translational modifications, reactant concentrations and allosteric effectors. Such multimodal regulations may be a general mechanism for metabolic modulation.
(© 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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