Protein-Sized Bright Fluorogenic Nanoparticles Based on Cross-Linked Calixarene Micelles with Cyanine Corona.

Autor: Shulov I; Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, Route du Rhin, 74, Illkirch, 67401 Cedex, France.; Organic Chemistry Department, Chemistry Faculty, Taras Shevchenko National University of Kyiv, 01033, Kyiv, Ukraine., Rodik RV; Institute of Organic Chemistry, National Academy of Science of Ukraine, 02660, Kyiv, Ukraine., Arntz Y; Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, Route du Rhin, 74, Illkirch, 67401 Cedex, France., Reisch A; Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, Route du Rhin, 74, Illkirch, 67401 Cedex, France., Kalchenko VI; Institute of Organic Chemistry, National Academy of Science of Ukraine, 02660, Kyiv, Ukraine., Klymchenko AS; Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, Route du Rhin, 74, Illkirch, 67401 Cedex, France.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2016 Dec 19; Vol. 55 (51), pp. 15884-15888. Date of Electronic Publication: 2016 Nov 16.
DOI: 10.1002/anie.201609138
Abstrakt: The key challenge in the field of fluorescent nanoparticles (NPs) for biological applications is to achieve superior brightness for sizes equivalent to single proteins (3-7 nm). We propose a concept of shell-cross-linked fluorescent micelles, in which PEGylated cyanine 3 and 5 bis-azides form a covalently attached corona on micelles of amphiphilic calixarene bearing four alkyne groups. The fluorescence quantum yield of the obtained monodisperse NPs, with a size of 7 nm, is a function of viscosity and reached up to 15 % in glycerol. In the on-state they are circa 2-fold brighter than quantum dots (QD-585), which makes them the smallest PEGylated organic NPs of this high brightness. FRET between cyanine 3 and 5 cross-linkers at the surface of NPs suggests their integrity in physiological media, organic solvents, and living cells, in which the NPs rapidly internalize, showing excellent imaging contrast. Calixarene micelles with a cyanine corona constitute a new platform for the development of protein-sized ultrabright fluorescent NPs.
(© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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