Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca 2+ -regulated Photoproteins of Different Organisms.

Autor: Eremeeva EV; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., Bartsev SI; Theoretical Biophysics Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia., van Berkel WJ; Laboratory of Biochemistry, Wageningen University & Research, Wageningen, The Netherlands., Vysotski ES; Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Krasnoyarsk, Russia.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2017 Mar; Vol. 93 (2), pp. 495-502. Date of Electronic Publication: 2016 Dec 16.
DOI: 10.1111/php.12664
Abstrakt: Upon binding their metal ion cofactors, Ca 2+ -regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca 2+ -regulated photoproteins-aequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculata-demonstrate the same bioluminescent kinetics pattern. Based on these findings, for the first time we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca 2+ -regulated photoproteins.
(© 2016 The American Society of Photobiology.)
Databáze: MEDLINE
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