A simple and reliable analytical method based on HPLC-UV to determine oleanonic acid content in Chios gum mastic for quality control.

Autor: Jin Y; School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea., Zhao J; School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea., Jeong KM; School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea., Yoo DE; School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea., Han SY; School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea., Choi SY; Nutribiotech Co., Ltd., Seoul, 06248, Republic of Korea., Ko DH; Nutribiotech Co., Ltd., Seoul, 06248, Republic of Korea., Kim HJ; Nutribiotech Co., Ltd., Seoul, 06248, Republic of Korea., Sung NH; Nutribiotech Co., Ltd., Seoul, 06248, Republic of Korea., Lee J; School of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi, 16419, Republic of Korea. jlee0610@skku.edu.
Jazyk: angličtina
Zdroj: Archives of pharmacal research [Arch Pharm Res] 2017 Jan; Vol. 40 (1), pp. 49-56. Date of Electronic Publication: 2016 Nov 17.
DOI: 10.1007/s12272-016-0853-2
Abstrakt: A reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the determination of oleanonic acid (OA) content in Chios gum mastic (CGM). A simple method involving methanol extraction of CGM powder followed by basification and ether extraction was developed to isolate the triterpenic fraction including OA. The triterpenic fraction was chromatographed on a Phenomenex Gemini C 18 column (150 × 4.6 mm, 5 µm) under a simple gradient elution of a mobile phase containing acetonitrile and water at a flow rate of 1.0 mL min -1 . The detection wavelength was set at 210 nm. Good linearity was achieved in the range of 100.0-1000.0 μg mL -1 with r 2  > 0.9993, and the limit of quantification was 32.22 μg mL -1 . Accuracy measured at three concentration levels was in the range of 93.72-99.56%, while intra-day and inter-day precisions estimated using both OA standard and CGM samples were no more than 2.83 and 4.57% RSD, respectively. Finally, this method was applied to real CGM samples from various batches, revealing that the OA contents were between 88.13 and 100.83 μg mg -1 . These results suggest that the current method can be applied as an efficient analytical method for quality control of CGM.
Databáze: MEDLINE
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