Interleukin-4 and interferon-γ are possible allergic markers in pediatric patients with β-lactam hypersensitivity.

Autor: Mahmoud KH; Department of Pediatrics, College of Medicine, Qassim University, Buraidah, Saudi Arabia., Alzolibani AA; Department of Dermatology, College of Medicine, Qassim University, Buraidah, Saudi Arabia., Rasheed Z; Department of Medical Biochemistry, College of Medicine, Qassim University, Buraidah, Saudi Arabia., Farouk Y; Department of Pediatrics, College of Medicine, Qassim University, Buraidah, Saudi Arabia., Saif GB; Department of Dermatology, College of Medicine, King Saud University, Riyadh, Saudi Arabia., Al Robaee AA; Department of Dermatology, College of Medicine, Qassim University, Buraidah, Saudi Arabia.
Jazyk: angličtina
Zdroj: International journal of applied & basic medical research [Int J Appl Basic Med Res] 2016 Oct-Dec; Vol. 6 (4), pp. 276-281.
DOI: 10.4103/2229-516X.192595
Abstrakt: Background: β-lactam agents are known to elicit T-cell-mediated immune responses that play a central role in the onset of allergic reactions, but the involvement of specific type of cytokines in drug allergy remains largely unexplored in humans.
Objectives: This study was undertaken to investigate the role of cytokines involvement in pediatric patients with β-lactam hypersensitivity and to determine whether involvement of cytokines in drug-mediated reactions are important for the perspective of allergic patient's management.
Methods: β-lactam-induced hypersensitivity reactions in eighty pediatric patients were determined by clinical manifestations and skin prick or intradermal testing. Production of T-helper (Th) type-1 cytokine interferon (INF)-γ, Th-2 cytokine interleukin (IL)-4, regulatory T-cell cytokine IL-10, and other cytokines IL-6 and IL-12 were determined by sandwich ELISAs.
Results: Diagnosis of β-lactam allergy was confirmed in 53 pediatric patients. IL-4 secretion in patients' sera was significantly higher as compared with healthy controls ( P < 0.05). However, INF-γ level in patients' sera was significantly lower as compared with controls ( P < 0.05). No significant alterations were found in the protein secretion of IL-10, IL-12, and IL-6 in allergic patients as compared with controls ( P > 0.05).
Conclusion: We conclude that IL-4 is specific marker for the diagnosis of β-lactam-induced hypersensitivity. Moreover, IL-4 in combination with INF-γ is more sensitive for the diagnosis of these reactions. This study also concludes that both IL-4 and INF-γ may play an active role in the onset of allergic reactions against β-lactam antibiotics.
Databáze: MEDLINE
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