Antimicrobial, antitrypanosomal and antibiofilm activity of Equisetum hyemale.

Autor: Dos Santos Alves CF; Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., Bonez PC; Department of Clinical and Toxicological Analysis, Mycobacteriology Laboratory, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., de Souza ME; Laboratory of Microbiological Research, Centro Universitário Franciscano (UNIFRA), Santa Maria, Brazil., da Cruz RC; Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., Boligon AA; Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., Piana M; Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., Brum TF; Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., Rossi GG; Department of Clinical and Toxicological Analysis, Mycobacteriology Laboratory, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., Jesus RD; Department of Industrial Pharmacy, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., Grando TH; Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., Monteiro SG; Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil., Anraku de Campos MM; Department of Clinical and Toxicological Analysis, Mycobacteriology Laboratory, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil., Giongo JL; Laboratory of Pharmacotechnique, Universidade Regional Integrada, URI-Santiago, Santiago, Brazil., Vianna Santos RC; Oral Microbiology Research Laboratory, Microbiology and Parasitology Department, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2016 Dec; Vol. 101, pp. 119-125. Date of Electronic Publication: 2016 Nov 14.
DOI: 10.1016/j.micpath.2016.11.008
Abstrakt: This study evaluates, for the first time, the antibiofilm, antimicrobial and antiparasitic potential of crude extract and fractions of stems of Equisetum hyemale against several infectious agents (bacteria, fungi, Mycobacterium and Trypanosomes) by broth microdilution technique and investigates the phenolic composition of the plant by high performance liquid chromatography. The crude extract and fractions showed antimicrobial activity, as they were capable of inhibiting the growth of bacteria in minimal inhibitory concentrations (MICs) ranging from 52.4 mg/mL to 3.27 mg/mL. For Candida species, the MICs ranged from 52.4 mg/mL to 6.5 mg/mL, and for Mycobacterium species from 2.5 mg/mL to 0.625 mg/mL. The dichloromethane fraction was able to reduce 83% of Pseudomonas aeruginosa biofilm formation and 51% of Candida albicans biofilms. The n-butanol fraction presents an important protozoal effect, reducing 100% of Trypanosoma evansi trypomastigotes after 9 h of exposure. The HPLC analysis revealed that the major substances are rosmarinic acid in dichloromethane fraction (7.38 ± 0.08 mg/g FS) and chlorogenic acid in ethyl acetate fraction (8.4 ± 0.26 mg/g FS). The crude extract and fractions of E. hyemale can be both useful and effective agents as a sustainable alternative for the treatment and prevention of several infectious agents.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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