X-ray microanalytical studies of mineral elements in the tripartite symbiosis between lima bean, N 2 -fixing bacteria and mycorrhizal fungi.

Autor: Rodak BW; Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil., Freitas DS; Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil. Electronic address: doug20106@gmail.com., Bamberg SM; Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil., Carneiro MA; Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil., Guilherme LR; Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2017 Jan; Vol. 132, pp. 14-20. Date of Electronic Publication: 2016 Nov 09.
DOI: 10.1016/j.mimet.2016.11.006
Abstrakt: The symbiosis between legumes, arbuscular mycorrhizal (AM) fungi, and N 2 -fixing bacteria (NFB) provides mutual nutritional gains. However, assessing the nutritional status of the microorganisms is a difficult task. A methodology that could assess this status, in situ, could assist managing these organisms in agriculture. This study used X-ray microanalyses to quantify and locate mineral elements in structures formed in a tripartite symbiosis. Lima bean (Phaseolus lunatus L. Walp) was cultivated in pots under greenhouse conditions, to which we have added AM fungal isolates (Glomus macrocarpum and Acaulospora colombiana) and NFB (Bradyrhizobium japonicum) inocula. Uninoculated control plants were also included. Symbionts were evaluated at the onset of flowering. Quantification of the mineral elements in the symbiotic components was performed using energy dispersive X-ray spectroscopy (EDX) and a scanning electron microscopy (SEM) was used to identify structures. EDX analysis detected 13 elements with the most abundant being N, Ca, and Se, occurring in all tissues, Fe in roots, Ni and Al in epidermis and P and Mo in nodules. Elemental quantification in fungal structures was not possible. The distribution of elements was related to their symbiotic function. X-ray microanalysis can be efficiently applied for nutritional diagnosis in tripartite symbiosis.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: