Pulmonary immune responses against Aspergillus fumigatus are characterized by high frequencies of IL-17 producing T-cells.

Autor: Jolink H; Department of Hematology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands; Department of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands. Electronic address: h.jolink@lumc.nl., de Boer R; Department of Hematology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Hombrink P; Sanquin Research and Landsteiner Laboratory, 1066 CX Amsterdam, The Netherlands., Jonkers RE; Department of Respiratory Medicine, Academic Medical Centre, 1105 AZ Amsterdam, The Netherlands., van Dissel JT; Department of Infectious Diseases, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Falkenburg JH; Department of Hematology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Heemskerk MH; Department of Hematology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: The Journal of infection [J Infect] 2017 Jan; Vol. 74 (1), pp. 81-88. Date of Electronic Publication: 2016 Nov 09.
DOI: 10.1016/j.jinf.2016.10.010
Abstrakt: In healthy individuals and in patients with invasive aspergillosis, Aspergillus-specific T-cells in peripheral blood display mainly a Thelper1 phenotype. Although in other fungal infections Thelper17 immunity is important, it was suggested that in aspergillus infection Thelper17 cells do not play a role or may even be detrimental.
Objectives: To compare the cytokine profiles of Aspergillus-specific CD4+ T-cells in peripheral blood and in the lung. To investigate the Thelper phenotype at the primary location of A. fumigatus exposure.
Methods: Lung-derived T-cells and peripheral blood T-cells from COPD-patients were stimulated with overlapping peptides of 6 A. fumigatus proteins. Aspergillus-specific T-cells were identified on the basis of the activation marker CD154 and production of TNFα. In addition, production of the cytokines IFNγ, IL-17, IL-4 and IL-5 by the Aspergillus-specific T-cells was measured.
Results: The majority of lung-derived Aspergillus-specific T-cells displayed a Thelper17 phenotype, and only low percentages of cells produced IFNγ. In contrast, in the peripheral blood of COPD patients Aspergillus-specific T-cells displayed a Thelper1 phenotype, similar as peripheral blood-derived Aspergillus-specific T-cells from healthy individuals.
Conclusions: These data demonstrate that in A. fumigatus infection, similar as in other fungal infections, Thelper17 cells may play a more important role in the immune response than was appreciated until now.
(Copyright © 2016 The British Infection Association. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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