miR-137 modulates coelomocyte apoptosis by targeting 14-3-3ζ in the sea cucumber Apostichopus japonicus.

Autor: Lv M; School of Marine Sciences, Ningbo University, China., Chen H; School of Marine Sciences, Ningbo University, China., Shao Y; School of Marine Sciences, Ningbo University, China., Li C; School of Marine Sciences, Ningbo University, China. Electronic address: lichenghua@nbu.edu.cn., Xu W; Louisiana State University, Agricultural Center, USA., Zhang W; School of Marine Sciences, Ningbo University, China., Zhao X; School of Marine Sciences, Ningbo University, China., Duan X; School of Marine Sciences, Ningbo University, China.
Jazyk: angličtina
Zdroj: Developmental and comparative immunology [Dev Comp Immunol] 2017 Feb; Vol. 67, pp. 86-96. Date of Electronic Publication: 2016 Nov 07.
DOI: 10.1016/j.dci.2016.11.008
Abstrakt: MicroRNAs (miRNAs) have emerged as key regulators in the host immune response and play a pivotal role in host-pathogen interactions by suppressing the transcriptional and post-transcriptional expression of target genes. miR-137, a well-documented tumor repressor, was previously found by high-throughput sequencing to be differentially expressed in diseased specimens of the sea cucumber Apostichopus japonicus. In this study, we identified 14-3-3ζ protein (Aj14-3-3ζ) as a novel target of miR-137 using isobaric tags for relative and absolute quantification (iTRAQ) and transcriptome screening. Expression analysis indicated that consistently depressed expression profiles of miR-137 and Aj14-3-3ζ were detected in both LPS-exposed primary coelomocytes and Vibrio splendidus-challenged sea cucumbers, suggesting a positive regulatory interaction. Consistently, miR-137 overexpression or inhibition in vitro and in vivo showed no effect on Aj14-3-3ζ mRNA levels, but the concentration of Aj14-3-3ζ protein was induced or repressed, respectively. Moreover, siRNA-mediated Aj14-3-3ζ knockdown in vivo decreased both mRNA and protein expression levels of Aj14-3-3ζ and significantly promoted coelomocyte apoptosis as assessed by flow cytometry, consistent with miR-137 inhibition. Overall, these results enhance our understanding of miR-137 regulatory roles in sea cucumber pathogenesis.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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