The Salivary Microbiome and Oral Cancer Risk: a Pilot Study in Fanconi Anemia.

Autor:	Furquim CP; 1 Graduate Program in Dentistry, Federal University of Paraná, Curitiba, PR, Brazil., Soares GM; 2 Department of Stomatology, Federal University of Paraná, Curitiba, PR, Brazil., Ribeiro LL; 3 Bone Marrow Transplantation Unit, Hospital de Clínicas, Federal University of Paraná, Curitiba, PR, Brazil., Azcarate-Peril MA; 4 Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Butz N; 4 Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Roach J; 5 Department of Research Computing, University of North Carolina, Chapel Hill, NC, USA., Moss K; 6 Department of Dental Ecology, School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Bonfim C; 3 Bone Marrow Transplantation Unit, Hospital de Clínicas, Federal University of Paraná, Curitiba, PR, Brazil., Torres-Pereira CC; 2 Department of Stomatology, Federal University of Paraná, Curitiba, PR, Brazil., Teles FR; 7 Department of Periodontology, University of North Carolina at Chapel Hill, School of Dentistry, Chapel Hill, NC, USA.
Jazyk:	angličtina
Zdroj:	Journal of dental research [J Dent Res] 2017 Mar; Vol. 96 (3), pp. 292-299. Date of Electronic Publication: 2016 Nov 13.
DOI:	10.1177/0022034516678169
Abstrakt:	Fanconi anemia (FA) is a rare genetic disease characterized by chromosomal instability and impaired DNA damage repair. FA patients develop oral squamous cell carcinoma (OSCC) earlier and more frequently than the general population, especially after hematopoietic stem cell transplantation (HSCT). Although evidence of an etiological role of the local microbiome and carcinogenesis has been mounting, no information exists regarding the oral microbiome of FA patients. The aim of this study was to explore the salivary microbiome of 61 FA patients regarding their oral health status and OSCC risk factors. After answering a questionnaire and receiving clinical examination, saliva samples were collected and analyzed using 16S rRNA sequencing of the V3-V4 hypervariable region. The microbial profiles associated with medical and clinical parameters were analyzed using general linear models. Patients were young (mean age, 22 y) and most had received HSCT ( n = 53). The most abundant phyla were Firmicutes [mean relative abundance (SD), 42.1% (10.1%)] and Bacteroidetes [(25.4% (11.4%)]. A history of graft-versus-host disease (GVHD) ( n = 27) was associated with higher proportions of Firmicutes (43.8% × 38.5%, P = 0.05). High levels of gingival bleeding were associated with the genera Prevotella (22.25% × 20%), Streptococcus (19.83% × 17.61%), Porphyromonas (3.63% × 1.42%, P = 0.03), Treponema (1.02% × 0.28%, P = 0.009), Parvimonas (0.28% × 0.07%, P = 0.02) and Dialister (0.27% × 0.10%, P = 0.04). Finally, participants transplanted over 11 y ago showed the highest levels of Streptococcus (18.4%), Haemophilus (12.7%) and Neisseria (6.8%). In conclusion, FA patients that showed poor oral hygiene harbored higher proportions of the genera of bacteria compatible with gingival disease. Specific microbial differences were associated with a history of oral GVHD and a history of oral mucositis. Competing Interests: The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.
Databáze:	MEDLINE
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