Quantification and genome-wide mapping of DNA double-strand breaks.

Autor: Grégoire MC; Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Quebec, Canada., Massonneau J; Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Quebec, Canada., Leduc F; Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Quebec, Canada., Arguin M; Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Quebec, Canada., Brazeau MA; Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Quebec, Canada., Boissonneault G; Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Quebec, Canada. Electronic address: guylain.boissonneault@usherbrooke.ca.
Jazyk: angličtina
Zdroj: DNA repair [DNA Repair (Amst)] 2016 Dec; Vol. 48, pp. 63-68. Date of Electronic Publication: 2016 Oct 29.
DOI: 10.1016/j.dnarep.2016.10.005
Abstrakt: DNA double-strand breaks (DSBs) represent a major threat to the genetic integrity of the cell. Knowing both their genome-wide distribution and number is important for a better assessment of genotoxicity at a molecular level. Available methods may have underestimated the extent of DSBs as they are based on markers specific to those undergoing active repair or may not be adapted for the large diversity of naturally occurring DNA ends. We have established conditions for an efficient first step of DNA nick and gap repair (NGR) allowing specific determination of DSBs by end labeling with terminal transferase. We used DNA extracted from HeLa cells harboring an I-SceI cassette to induce a targeted nick or DSB and demonstrated by immunocapture of 3'-OH that a prior step of NGR allows specific determination of loci-specific or genome wide DSBs. This method can be applied to the global determination of DSBs using radioactive end labeling and can find several applications aimed at understanding the distribution and kinetics of DSBs formation and repair.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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