| Autor: |
Ko EY; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea., Yoon WJ; Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, 699-943, Republic of Korea., Lee HW; World Institute of Kimchi, Gwangju 503-360, Republic of Korea., Heo SJ; Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju, 63349, Republic of Korea., Ko YH; Department of Food Bioengineering, Jeju National University, Jeju 690-756, Republic of Korea., Fernando IP; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea., Cho K; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea., Lee CH; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea., Hur SP; Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju, 63349, Republic of Korea., Cho SH; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea., Ahn G; Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 550-74, Republic Korea., Kim D; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea., Kim KN; Jeju Center, Korea Basic Science Institute (KBSI), Jeju 690-140, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon 305-350, Republic of Korea. |
| Abstrakt: |
The anti-inflammatory properties of the supercritical fluid extract of Ishige okamurae (SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it's anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E 2 (PGE 2 ), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 production in LPS-stimulated RAW 264.7 cells, without inducing cytotoxicity. SFEIO did not effect on the LPS-induced p38 kinase phosphorylation, whereas it attenuated the extracellular-related signaling kinase (ERK) and c-Jun N-terminal kinase (JNK) phosphorylation. Furthermore, SFEIO inhibited the LPS-induced IκB-α degradation and p50 NF-κB activation. These results suggest that SFEIO exerts its anti-inflammatory effects in LPS-activated RAW 264.7 cells by down-regulating the activation of ERK, JNK, and NF-κB. |
