Senescence Phenotypes Induced by Ras in Primary Cells.

Autor:	Lau L; Department of Biochemistry and Molecular Pharmacology, Perlmutter Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY, 10016, USA., David G; Department of Biochemistry and Molecular Pharmacology, Perlmutter Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY, 10016, USA. gregory.david@ynyumc.org.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1534, pp. 17-30.
DOI:	10.1007/978-1-4939-6670-7_2
Abstrakt:	Cellular senescence is defined as a state of stable cell-cycle arrest that is distinct from quiescence and terminal differentiation. Many stimuli can induce senescence, including telomere shortening and oncogene activation. The phenotypes elicited by pro-senescent signals can be heterogeneous depending on the stimulus and the cell type affected. To date, there is not a definitive marker that can ubiquitously and specifically mark all senescent cells. Therefore, several independent markers must be utilized to ascertain the senescent state of a cell or group of cells. Here, we describe common assays used to assess oncogenic Ras-induced senescence.
Databáze:	MEDLINE
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