Comparison and analysis of Wuding and avian chicken skeletal muscle satellite cells.

Autor:	Tong HQ; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China., Jiang ZQ; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China., Dou TF; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China., Li QH; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China., Xu ZQ; Department of Food Science, Yunnan Agricultural University, Kunming, Yunnan Province, China., Liu LX; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China., Gu DH; Department of Food Science, Yunnan Agricultural University, Kunming, Yunnan Province, China., Rong H; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China., Huang Y; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China., Chen XB; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China., Jois M; School of Life Sciences Faculty of Science, Technology and Engineering La Trobe University, Bundoora, Victoria, Australia., Te Pas MF; Animal Breeding and Genetics Centre, Wageningen UR Livestock Science, Wageningen, The Netherlands., Ge CR; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China gcrzal@126.com., Jia JJ; Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University, Kunming, Yunnan Province, China.
Jazyk:	angličtina
Zdroj:	Genetics and molecular research : GMR [Genet Mol Res] 2016 Oct 05; Vol. 15 (4). Date of Electronic Publication: 2016 Oct 05.
DOI:	10.4238/gmr.15048815
Abstrakt:	Chicken skeletal muscle satellite cells are located between the basement membrane and the sarcolemma of mature muscle fibers. Avian broilers have been genetically selected based on their high growth velocity and large muscle mass. The Wuding chicken is a famous local chicken in Yunnan Province that undergoes non-selection breeding and is slow growing. In this study, we aimed to explore differences in the proliferation and differentiation properties of satellite cells isolated from the two chicken breeds. Using immunofluorescence, hematoxylin-eosin staining and real-time polymerase chain reaction analysis, we analyzed the in vitro characteristics of proliferating and differentiating satellite cells isolated from the two chicken breeds. The growth curve of satellite cells was S-shaped, and cells from Wuding chickens entered the logarithmic phase and plateau phase 1 day later than those from Avian chicken. The results also showed that the two skeletal muscle satellite cell lines were positive for Pax7, MyoD and IGF-1. The expression of Pax7 followed a downward trend, whereas that of MyoD and IGF-1 first increased and subsequently decreased in cells isolated from the two chickens. These data indicated that the skeletal muscle satellite cells of Avian chicken grow and differentiate faster than did those of Wuding chickens. We suggest that the methods of breeding selection applied to these breeds regulate the characteristics of skeletal muscle satellite cells to influence muscle growth.
Databáze:	MEDLINE
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