The Microminipig as an Animal Model for Influenza A Virus Infection.

Autor:	Iwatsuki-Horimoto K; Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan., Nakajima N; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan., Shibata M; Swine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, Kikugawa, Shizuoka, Japan., Takahashi K; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan., Sato Y; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan., Kiso M; Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan., Yamayoshi S; Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan., Ito M; Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan., Enya S; Swine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, Kikugawa, Shizuoka, Japan., Otake M; Swine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, Kikugawa, Shizuoka, Japan., Kangawa A; Swine and Poultry Research Center, Shizuoka Prefectural Research Institute of Animal Industry, Kikugawa, Shizuoka, Japan., da Silva Lopes TJ; Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA., Ito H; Ito Business Planning, Hamamatsu, Shizuoka, Japan., Hasegawa H; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan., Kawaoka Y; Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan kawaoka@ims.u-tokyo.ac.jp.; Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.; Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Jazyk:	angličtina
Zdroj:	Journal of virology [J Virol] 2017 Jan 03; Vol. 91 (2). Date of Electronic Publication: 2017 Jan 03 (Print Publication: 2017).
DOI:	10.1128/JVI.01716-16
Abstrakt:	Pigs are considered a mixing vessel for the generation of novel pandemic influenza A viruses through reassortment because of their susceptibility to both avian and human influenza viruses. However, experiments to understand reassortment in pigs in detail have been limited because experiments with regular-sized pigs are difficult to perform. Miniature pigs have been used as an experimental animal model, but they are still large and require relatively large cages for housing. The microminipig is one of the smallest miniature pigs used for experiments. Introduced in 2010, microminipigs weigh around 10 kg at an early stage of maturity (6 to 7 months old) and are easy to handle. To evaluate the microminipig as an animal model for influenza A virus infection, we compared the receptor distribution of 10-week-old male pigs (Yorkshire Large White) and microminipigs. We found that both animals have SAα2,3Gal and SAα2,6Gal in their respiratory tracts, with similar distributions of both receptor types. We further found that the sensitivity of microminipigs to influenza A viruses was the same as that of larger miniature pigs. Our findings indicate that the microminipig could serve as a novel model animal for influenza A virus infection. Importance: The microminipig is one of the smallest miniature pigs in the world and is used as an experimental animal model for life science research. In this study, we evaluated the microminipig as a novel animal model for influenza A virus infection. The distribution of influenza virus receptors in the respiratory tract of the microminipig was similar to that of the pig, and the sensitivity of microminipigs to influenza A viruses was the same as that of miniature pigs. Our findings suggest that microminipigs represent a novel animal model for influenza A virus infection. (Copyright © 2017 American Society for Microbiology.)
Databáze:	MEDLINE
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