Transcriptional Analysis of serk1 and serk3 Coreceptor Mutants.

Autor:	van Esse GW; Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands., Ten Hove CA; Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands., Guzzonato F; Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands., van Esse HP; Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands., Boekschoten M; Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands., Ridder L; Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands., Vervoort J; Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands., de Vries SC; Laboratory of Biochemistry, Wageningen University, 6708 WE Wageningen, The Netherlands sacco.devries@wur.nl.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2016 Dec; Vol. 172 (4), pp. 2516-2529. Date of Electronic Publication: 2016 Nov 01.
DOI:	10.1104/pp.16.01478
Abstrakt:	Somatic embryogenesis receptor kinases (SERKs) are ligand-binding coreceptors that are able to combine with different ligand-perceiving receptors such as BRASSINOSTEROID INSENSITIVE1 (BRI1) and FLAGELLIN-SENSITIVE2. Phenotypical analysis of serk single mutants is not straightforward because multiple pathways can be affected, while redundancy is observed for a single phenotype. For example, serk1serk3 double mutant roots are insensitive toward brassinosteroids but have a phenotype different from bri1 mutant roots. To decipher these effects, 4-d-old Arabidopsis (Arabidopsis thaliana) roots were studied using microarray analysis. A total of 698 genes, involved in multiple biological processes, were found to be differentially regulated in serk1-3serk3-2 double mutants. About half of these are related to brassinosteroid signaling. The remainder appear to be unlinked to brassinosteroids and related to primary and secondary metabolism. In addition, methionine-derived glucosinolate biosynthesis genes are up-regulated, which was verified by metabolite profiling. The results also show that the gene expression pattern in serk3-2 mutant roots is similar to that of the serk1-3serk3-2 double mutant roots. This confirms the existence of partial redundancy between SERK3 and SERK1 as well as the promoting or repressive activity of a single coreceptor in multiple simultaneously active pathways. (© 2016 American Society of Plant Biologists. All Rights Reserved.)
Databáze:	MEDLINE
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