Biosynthesis of poly(3-hydroxybutyrateco-3-hydroxy-4-methylvalerate) by Strain Azotobacter chroococcum 7B.

Autor: Bonartsev AP; Faculty of Biology, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234 , Russia ; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow, 119071, Russia., Bonartseva GA; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow, 119071, Russia., Myshkina VL; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow, 119071, Russia., Voinova VV; Faculty of Biology, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234 , Russia., Mahina TK; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow, 119071, Russia., Zharkova II; Faculty of Biology, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234 , Russia., Yakovlev SG; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow, 119071, Russia., Zernov AL; Faculty of Biology, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234 , Russia., Ivanova EV; Faculty of Biology, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234 , Russia., Akoulina EA; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow, 119071, Russia., Kuznetsova ES; Faculty of Biology, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234 , Russia., Zhuikov VA; A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., Moscow, 119071, Russia., Alekseeva SG; JSC «Institute of Plastics», Petrovskiy proezd, 35, Moscow, 111024, Russia., Podgorskii VV; Federal scientific-clinical center of physics-chemical medicine, Federal medical-biological agency, Malaya Pirogovskaya str., 1a, Moscow, 119435, Russia., Bessonov IV; Bauman Moscow State Technical University, 5, 2-nd Baumanskaya, Moscow, 105005, Russia., Kopitsyna MN; Bauman Moscow State Technical University, 5, 2-nd Baumanskaya, Moscow, 105005, Russia., Morozov AS; Bauman Moscow State Technical University, 5, 2-nd Baumanskaya, Moscow, 105005, Russia., Milanovskiy EY; Faculty of Soil Science, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234, Russia., Tyugay ZN; Faculty of Soil Science, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234, Russia., Bykova GS; Faculty of Soil Science, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234, Russia., Kirpichnikov MP; Faculty of Biology, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234 , Russia., Shaitan KV; Faculty of Biology, M.V.Lomonosov Moscow State University, Leninskie gory, 1-12, Moscow, 119234 , Russia.
Jazyk: angličtina
Zdroj: Acta naturae [Acta Naturae] 2016 Jul-Sep; Vol. 8 (3), pp. 77-87.
Abstrakt: Production of novel polyhydroxyalkanoates (PHAs), biodegradable polymers for biomedical applications, and biomaterials based on them is a promising trend in modern bioengineering. We studied the ability of an effective strain-producer Azotobacter chroococcum 7B to synthesize not only poly(3-hydroxybutyrate) homopolymer (PHB) and its main copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), but also a novel copolymer, poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) (PHB4MV). For the biosynthesis of PHB copolymers, we used carboxylic acids as additional carbon sources and monomer precursors in the chain of synthesized copolymers. The main parameters of these polymers' biosynthesis were determined: strain-producer biomass yield, polymer yield, molecular weight and monomer composition of the synthesized polymers, as well as the morphology of A. chroococcum 7B bacterial cells. The physico-chemical properties of the polymers were studied using nuclear magnetic resonance spectroscopy (NMR), differential scanning calorimetry (DSC), contact angle test, and other methods. In vitro biocompatibility of the obtained polymers was investigated using stromal cells isolated from the bone marrow of rats with the XTT cell viability test. The synthesis of the novel copolymer PHB4MV and its chemical composition were demonstrated by NMR spectroscopy: the addition of 4-methylvaleric acid to the culture medium resulted in incorporation of 3-hydroxy-4-methylvalerate (3H4MV) monomers into the PHB polymer chain (0.6 mol%). Despite the low molar content of 3H4MV in the obtained copolymer, its physico-chemical properties were significantly different from those of the PHB homopolymer: it has lower crystallinity and a higher contact angle, i.e. the physico-chemical properties of the PHB4MV copolymer containing only 0.6 mol% of 3H4MV corresponded to a PHBV copolymer with a molar content ranging from 2.5% to 7.8%. In vitro biocompatibility of the obtained PHB4MV copolymer, measured in the XTT test, was not statistically different from the cell growth of PHB and PHBV polymers, which make its use possible in biomedical research and development.
Databáze: MEDLINE
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