Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy.
| Autor: | Cortés E, Huidobro PA, Sinclair HG, Guldbrand S, Peveler WJ; Department of Chemistry, University College London , 20 Gordon Street, London WC1H 0AJ, United Kingdom., Davies T, Parrinello S, Görlitz F, Dunsby C, Neil MA, Sivan Y; Unit of Electro-optics Engineering, Ben-Gurion University , Beer-Sheba 8410501, Israel., Parkin IP; Department of Chemistry, University College London , 20 Gordon Street, London WC1H 0AJ, United Kingdom., French PM, Maier SA |
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| Jazyk: | angličtina |
| Zdroj: | ACS nano [ACS Nano] 2016 Nov 22; Vol. 10 (11), pp. 10454-10461. Date of Electronic Publication: 2016 Nov 03. |
| DOI: | 10.1021/acsnano.6b06361 |
| Abstrakt: | Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm. These particles provide an enhancement of up to 50% of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. The nanoparticle-assisted STED probes reported here represent a ∼2 × 10 3 reduction in probe volume compared to previously used nanoparticles. Finally, we demonstrate their application toward plasmon-assisted STED cellular imaging at low-depletion powers, and we also discuss their current limitations. |
| Databáze: | MEDLINE |
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