Performing Repeated Quantitative Small-Animal PET with an Arterial Input Function Is Routinely Feasible in Rats.

Autor: Huang CC; School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.; Department of Surgery, Cathay General Hospital, Taipei, Taiwan., Wu CH; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan., Huang YY; Department of Nuclear Medicine, National Taiwan University Hospital, Taipei, Taiwan; and., Tzen KY; Department of Nuclear Medicine, National Taiwan University Hospital, Taipei, Taiwan; and., Chen SF; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.; Department of Physical Medicine and Rehabilitation, Cheng Hsin General Hospital, Taipei, Taiwan., Tsai ML; School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan., Wu HM; School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan cwu22713@gmail.com.
Jazyk: angličtina
Zdroj: Journal of nuclear medicine : official publication, Society of Nuclear Medicine [J Nucl Med] 2017 Apr; Vol. 58 (4), pp. 611-616. Date of Electronic Publication: 2016 Oct 27.
DOI: 10.2967/jnumed.116.182402
Abstrakt: Performing quantitative small-animal PET with an arterial input function has been considered technically challenging. Here, we introduce a catheterization procedure that keeps a rat physiologically stable for 1.5 mo. We demonstrated the feasibility of quantitative small-animal 18 F-FDG PET in rats by performing it repeatedly to monitor the time course of variations in the cerebral metabolic rate of glucose (CMR glc ). Methods: Aseptic surgery was performed on 2 rats. Each rat underwent catheterization of the right femoral artery and left femoral vein. The catheters were sealed with microinjection ports and then implanted subcutaneously. Over the next 3 wk, each rat underwent 18 F-FDG quantitative small-animal PET 6 times. The CMR glc of each brain region was calculated using a 3-compartment model and an operational equation that included a k* 4 Results: On 6 mornings, we completed 12 18 F-FDG quantitative small-animal PET studies on 2 rats. The rats grew steadily before and after the 6 quantitative small-animal PET studies. The CMR glc of the conscious brain (e.g., right parietal region, 99.6 ± 10.2 μmol/100 g/min; n = 6) was comparable to that for 14 C-deoxyglucose autoradiographic methods. Conclusion: Maintaining good blood patency in catheterized rats is not difficult. Longitudinal quantitative small-animal PET imaging with an arterial input function can be performed routinely.
(© 2017 by the Society of Nuclear Medicine and Molecular Imaging.)
Databáze: MEDLINE
Externí odkaz: