| Autor: |
Slaine PD; a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada., Ackford JG; a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada., Kropinski AM; a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada., Kozak RA; a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada., Krell PJ; b Department of Molecular and Cellular Biology, College of Biological Sciences, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada., Nagy É; a Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, 50 Stone Road East, ON N1G 2W1, Canada. |
| Abstrakt: |
Fowl aviadenoviruses, many of which are of importance in veterinary medicine, are classified into 5 species. In this study, a pathogenic isolate and a nonpathogenic isolate of fowl aviadenovirus serotype 11 (FAdV-11) of species Fowl aviadenovirus D were characterized. Growth rates were analyzed for the 2 isolates, showing notable differences. The complete genomic sequences of the viruses were fully determined and were analyzed. The genomes of the 2 isolates showed 98.1% sequence identity and revealed 6 nonsynonymous mutations between the Ontario isolates. Two of the 6 mutations were also found in the sequences of recently published pathogenic Chinese fowl aviadenovirus 11 isolates, suggesting potential molecular markers that could be associated with pathogenesis. Deletions were found in the L5 region within the overlapping coding sequences for the 100, 22, and 33 kDa proteins, and these were found in only the nonpathogenic isolates. This molecular pattern was identified in FAdV-9, another nonpathogenic FAdV-D species virus. Furthermore, the tandem repeat regions varied dramatically; the pathogenic isolates contained a reduced number of tandem repeats compared with the nonpathogenic isolates. Lastly, a protein produced early in infection was analyzed using bioinformatics to determine its role in disease. This study highlights several candidate molecular determinants of avian adenovirus genomes related to pathogenicity. |
