SANIST: optimization of a technology for compound identification based on the European Union directive with applications in forensic, pharmaceutical and food analyses.

Autor: Cristoni S; I.S.B.-Ion Source & Biotechnologies, Bresso, Milano, Italy., Dusi G; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, B. Ubertini, Brescia, Italy., Brambilla P; Desio Hospital, Desio, Milano, Italy., Albini A; IRCCS MultiMedica, Milano, Italy., Conti M; Sant'Orsola Malpighi Hospital, Bologna, Italy., Brambilla M; Desio Hospital, Desio, Milano, Italy., Bruno A; IRCCS MultiMedica, Milano, Italy., Di Gaudio F; CQRC - Quality Control laboratory and Chemical Risk, Department of Pathobiology and Medical Biotechnology (DIBIMED), University Hospital Palermo, Italy., Ferlin L; Sant'Anna Hospital, Como, Italy., Tazzari V; Laboratorio Unico AUSL della Romagna, Pievesestina di Cesena, Italy., Mengozzi S; Laboratorio Unico AUSL della Romagna, Pievesestina di Cesena, Italy., Barera S; I.S.B.-Ion Source & Biotechnologies, Bresso, Milano, Italy., Sialer C; RDI-Ilender pharmaceutical corporation, Lima, Peru.; PACT USS Scientific Technological Park University Señor de Sipan, Chiclayo, Peru., Trenti T; Baggiovara Hospital, Modena, Italy., Cantu M; Bellinzona Hospital, Bellinzona, Swizzerland., Rossi Bernardi L; IRCCS MultiMedica, Milano, Italy., Noonan DM; IRCCS MultiMedica, Milano, Italy.; Department of Biotechnologies Life Sciences, University of Insubria, Varese, Italy.
Jazyk: angličtina
Zdroj: Journal of mass spectrometry : JMS [J Mass Spectrom] 2017 Jan; Vol. 52 (1), pp. 16-21.
DOI: 10.1002/jms.3895
Abstrakt: Electrospray Ionization and collision induced dissociation tandem mass spectrometry are usually employed to obtain compound identification through a mass spectra match. Different algorithms have been developed for this purpose (for example the nist match algorithm). These approaches compare the tandem mass spectra of the unknown analyte with the tandem mass spectra spectra of known compounds inserted in a database. The compounds are usually identified on the basis of spectral match value associated with a probability of recognition. However, this approach is not usually applied to multiple reaction monitoring transition spectra achieved by means of triple quadrupole apparatus, mainly due to the lack of a transition spectra database. The Surface Activated Chemical Ionization-Electrospray-NIST Bayesian model database search (SANIST) platform has been recently developed for new potential metabolite biomarker discovery, to confirm their identity and to use them for clinical and diagnostic applications. Here, we present an improved version of the SANIST platform that extends its application to forensic, pharmaceutical, and food analysis studies, where the compound identification rules are strict. The European Union (EU) has set directives for compound identification (EU directive 2002/657/EC). We have applied the SANIST method to identification of 11-nor-9-carboxytetrahydro-cannabinol in urine samples (an example of a forensic application), circulating levels of the immunosuppressive drug tacrolimus in blood (an example of a pharmaceutical application) and glyphosate in fruit juice (an example of a food analysis application) that meet the EU directive requirements. Copyright © 2016 John Wiley & Sons, Ltd.
(Copyright © 2016 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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