Efficacy of a disinfectant containing silver dihydrogen citrate against GI.6 and GII.4 human norovirus.
| Autor: | Manuel CS; Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC, USA., Moore MD; Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC, USA., Jaykus LA; Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of applied microbiology [J Appl Microbiol] 2017 Jan; Vol. 122 (1), pp. 78-86. Date of Electronic Publication: 2016 Dec 06. |
| DOI: | 10.1111/jam.13331 |
| Abstrakt: | Aims: Human norovirus is a major public health burden and is resistant to numerous sanitizers and disinfectants. In this study, we tested the efficacy of an antimicrobial product containing a blend of silver ions and citric acid (silver dihydrogen citrate; SDC) against GI.6 and GII.4 HuNoV. Methods and Results: Pure ® hard surface disinfectant (Pure Bioscience, El Cajon, CA) was evaluated using ASTM International virucidal suspension and stainless steel carrier assays. The effect of SDC (or citrate alone) exposure on viral integrity was evaluated using RT-qPCR, transmission electron microscopy, sodium dodecyl sulphate-polyacrylamide gel electrophoresis/Western blot analysis and a receptor-binding assay. Suspension assays showed a 4·0 log Conclusions: Our results suggest that SDC acts exclusively on the viral capsid, and shows efficacy against HuNoV when used on precleaned surfaces. Significance and Impact of the Study: This study sheds light on the mechanisms and efficacy of a novel antimicrobial against HuNoV. Our results suggest: (i) silver ions exclusively target the viral capsid, and not the RNA genome; (ii) citrate is not crucial for HuNoV capsid deformation. (© 2016 The Society for Applied Microbiology.) |
| Databáze: | MEDLINE |
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