Occurrence of Blastocystis sp. in water catchments at Malay villages and Aboriginal settlement during wet and dry seasons in Peninsular Malaysia.

Autor:	Noradilah SA; Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Pandan Indah, Kuala Lumpur, Malaysia., Lee IL; Kulliyyah of Medicine and Health Sciences, Kolej Universiti Insaniah , Kuala Ketil , Kedah , Malaysia., Anuar TS; Department of Medical Laboratory Technology, Universiti Teknologi MARA, Puncak Alam, Selangor, Malaysia; Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor, Malaysia., Salleh FM; Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre , Cheras , Kuala Lumpur , Malaysia., Abdul Manap SN; Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre , Cheras , Kuala Lumpur , Malaysia., Mohd Mohtar NS; Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre , Cheras , Kuala Lumpur , Malaysia., Azrul SM; Multipurpose Laboratory, Universiti Kebangsaan Malaysia Medical Centre , Cheras , Kuala Lumpur , Malaysia., Abdullah WO; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia , Pandan Indah , Kuala Lumpur , Malaysia., Moktar N; Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre , Cheras , Kuala Lumpur , Malaysia.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2016 Oct 06; Vol. 4, pp. e2541. Date of Electronic Publication: 2016 Oct 06 (Print Publication: 2016).
DOI:	10.7717/peerj.2541
Abstrakt:	In the tropics, there are too few studies on isolation of Blastocystis sp. subtypes from water sources; in addition, there is also an absence of reported studies on the occurrence of Blastocystis sp. subtypes in water during different seasons. Therefore, this study was aimed to determine the occurrence of Blastocystis sp. subtypes in river water and other water sources that drained aboriginal vicinity of highly endemic intestinal parasitic infections during wet and dry seasons. Water samples were collected from six sampling points of Sungai Krau (K1-K6) and a point at Sungai Lompat (K7) and other water sources around the aboriginal villages. The water samples were collected during both seasons, wet and dry seasons. Filtration of the water samples were carried out using a flatbed membrane filtration system. The extracted DNA from concentrated water sediment was subjected to single round polymerase chain reaction and positive PCR products were subjected to sequencing. All samples were also subjected to filtration and cultured on membrane lactose glucuronide agar for the detection of faecal coliforms. During wet season, Blastocystis sp. ST1, ST2 and ST3 were detected in river water samples. Blastocystis sp. ST3 occurrence was sustained in the river water samples during dry season. However Blastocystis sp. ST1 and ST2 were absent during dry season. Water samples collected from various water sources showed contaminations of Blastocystis sp. ST1, ST2, ST3 and ST4, during wet season and Blastocystis sp. ST1, ST3, ST8 and ST10 during dry season. Water collected from all river sampling points during both seasons showed growth of Escherichia coli and Enterobacter aerogenes , indicating faecal contamination. In this study, Blastocystis sp. ST3 is suggested as the most robust and resistant subtype able to survive in any adverse environmental condition. Restriction and control of human and animal faecal contaminations to the river and other water sources shall prevent the transmission of Blastocystis sp. to humans and animals in this aboriginal community. Competing Interests: The authors declare there are no competing interests.
Databáze:	MEDLINE
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