High-throughput and simultaneous quantitative analysis of homocysteine-methionine cycle metabolites and co-factors in blood plasma and cerebrospinal fluid by isotope dilution LC-MS/MS.

Autor: Guiraud SP; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, CH-1015, Lausanne, Switzerland. seuping.guiraud@rd.nestle.com., Montoliu I; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, CH-1015, Lausanne, Switzerland., Da Silva L; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, CH-1015, Lausanne, Switzerland., Dayon L; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, CH-1015, Lausanne, Switzerland., Galindo AN; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, CH-1015, Lausanne, Switzerland., Corthésy J; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, CH-1015, Lausanne, Switzerland., Kussmann M; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, CH-1015, Lausanne, Switzerland., Martin FP; Nestlé Institute of Health Sciences SA, Campus EPFL, Innovation Park, CH-1015, Lausanne, Switzerland.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2017 Jan; Vol. 409 (1), pp. 295-305. Date of Electronic Publication: 2016 Oct 18.
DOI: 10.1007/s00216-016-0003-1
Abstrakt: The methionine cycle is a key pathway contributing to the regulation of human health, with well-established involvement in cardiovascular diseases and cognitive function. Changes in one-carbon cycle metabolites have also been associated with mild cognitive decline, vascular dementia, and Alzheimer's disease. Today, there is no single analytical method to monitor both metabolites and co-factors of the methionine cycle. To address this limitation, we here report for the first time a new method for the simultaneous quantitation of 17 metabolites in the methionine cycle, which are homocysteic acid, taurine, serine, cysteine, glycine, homocysteine, riboflavin, methionine, pyridoxine, cystathionine, pyridoxamine, S-adenosylhomocysteine, S-adenosylmethionine, betaine, choline, dimethylglycine, and 5-methyltetrahydrofolic acid. This multianalyte method, developed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), provides a highly accurate and precise quantitation of these 17 metabolites for both plasma and cerebrospinal fluid metabolite monitoring. The method requires a simple sample preparation, which, combined with a short chromatographic run time, ensures a high sample throughput. This analytical strategy will thus provide a novel metabolomics approach to be employed in large-scale observational and intervention studies. We expect such a robust method to be particularly relevant for broad and deep molecular phenotyping of individuals in relation to their nutritional requirements, health monitoring, and disease risk management.
Competing Interests: Compliance with ethical standards All plasma and CSF human samples were provided by PrecisionMed, Inc. (CA, USA, protocol 8009) and collected under IRB-approved protocols. Conflict of interest The authors declare that they have no competing interests.
Databáze: MEDLINE
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