Effects of the herbicide Roundup on the polychaeta Laeonereis acuta: Cholinesterases and oxidative stress.

Autor: de Melo Tarouco F; Universidade Federal do Rio Grande - FURG, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Ciências Fisiológicas, Brazil. Electronic address: fabiobio304@hotmail.com., de Godoi FGA; Universidade Federal do Rio Grande, Av. Itália km 8, 96203-900 Rio Grande, RS, Brazil. Electronic address: filipe_godoi92@hotmail.com., Velasques RR; Universidade Federal do Rio Grande - FURG, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Ciências Fisiológicas, Brazil. Electronic address: robson_velasques@yahoo.com.br., da Silveira Guerreiro A; Universidade Federal do Rio Grande - FURG, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Ciências Fisiológicas, Brazil. Electronic address: amandahg@gmail.com., Geihs MA; Instituto de Oceanografia, Universidade Federal do Rio Grande (IO-FURG). Electronic address: geihs@hotmail.com., da Rosa CE; Universidade Federal do Rio Grande - FURG, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Ciências Fisiológicas, Brazil. Electronic address: carlosrosa@furg.br.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2017 Jan; Vol. 135, pp. 259-266. Date of Electronic Publication: 2016 Oct 14.
DOI: 10.1016/j.ecoenv.2016.10.014
Abstrakt: Glyphosate based herbicides, including Roundup, are widely employed in agriculture and urban spaces. The objective of this study was to evaluate the toxicological effects of Roundup on the estuarine polychaeta Laeonereis acuta. Biomarkers of oxidative stress as well as acetylcholinesterase and propionilcholinesterase activities were analyzed. Firstly, the LC 50 96h for L. acuta was established (8.19mg/L). After, the animals were exposed to two Roundup concentrations: 3.25mg/L (non-observed effect concentration - NOEC) and 5.35mg/L (LC 10 ) for 24h and 96h. Oxygen consumption was determined and the animals were divided into three body regions (anterior, middle and posterior) for biochemical analysis. An inhibition of both cholinesterase isoforms were observed in animals exposed to both Roundup concentrations after 96h. A significant reactive oxygen species (ROS) reduction was observed in the posterior region of animals in both periods, while antioxidant capacity against peroxyl radicals (ACAP) was reduced in the posterior region of animals exposed for 24h. Considering the antioxidant defense system, both GSH levels and enzyme activities (catalase, superoxide dismutase, glutathione s-transferase, glutathione peroxidase and glutamate cysteine ligase) were not altered after exposure. Lipid peroxidation was reduced in all analyzed body regions in both Roundup concentrations after 24h. Animals exposed to the highest concentration presented a reduction in lipid peroxidation in the anterior region after 96h, while animals exposed to the lowest concentration presented a reduction in the middle region. Overall results indicate that Roundup exposure presents toxicity to L. acuta, causing a disruption in ROS and ACAP levels as well as affects the cholinergic system of this invertebrate species.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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