| Autor: |
Martin-Cano FE; Department of Physiology, Faculty of Nursing and Occupational Therapy, University of Extremadura, 10003, Caceres, Spain., Caso-Agundez M; Department of Physiology, Faculty of Nursing and Occupational Therapy, University of Extremadura, 10003, Caceres, Spain., Camello-Almaraz C; Department of Physiology, Faculty of Nursing and Occupational Therapy, University of Extremadura, 10003, Caceres, Spain., Santos FJ; Digestive Surgery Service, 'San Pedro de Alcantara' Hospital, 10003, Cáceres, Spain., Espin MT; Digestive Surgery Service, 'Infanta Cristina' Hospital, 06006, Badajoz, Spain., Madrid JA; Chronobiology Laboratory, College of Biology, University of Murcia, IMIB-Arrixaca, 30100, Murcia, Spain., Diez-Perez A; Musculoskeletal research group, Red Temática de Investigación Cooperativa en Envejecimiento y Fragilidad (RETICEF), ISCIII, IMIM (Hospital del Mar Research Institute), Barcelona, Spain., Camello PJ; Department of Physiology, Faculty of Nursing and Occupational Therapy, University of Extremadura, 10003, Caceres, Spain., Pozo MJ; Department of Physiology, Faculty of Nursing and Occupational Therapy, University of Extremadura, 10003, Caceres, Spain. mjpozo@unex.es. |
| Abstrakt: |
Urinary bladder function consists in the storage and controlled voiding of urine. Translational studies require animal models that match human characteristics, such as Octodon degus, a diurnal rodent. This study aims to characterize the contractility of the detrusor muscle and the morphology and code of the vesical plexus from O. degus. Body temperature was measured by an intra-abdominal sensor, the contractility of detrusor strips was evaluated by isometric tension recording, and the vesical plexus was studied by electrical field stimulation (EFS) and immunofluorescence. The animals showed a diurnal chronotype as judged from core temperature. The myogenic contractile response of the detrusor muscle to increasing doses of KCl reached its maximum (31.04 mN/mm 2 ) at 60 mM. In the case of cumulative dose-response of bethanecol, the maximum response (37.42 mN/mm 2 ) was reached at 3.2 × 10 -4 M. The response to ATP was clearly smaller (3.8 mN/mm 2 ). The pharmacological dissection of the EFS-induced contraction identified ACh and sensory fibers as the main contributors to this response. The neurons of the vesical plexus were located mainly in the trigone area, grouped in big and small ganglia. Out of them, 48.1 % of the neurons were nitrergic and 62.7 % cholinergic. Our results show functional and morphological similarities between the urinary bladder of O. degus and that of humans. |
Full text from SpringerLink