The poly(ADP-ribose)-dependent chromatin remodeler Alc1 induces local chromatin relaxation upon DNA damage.

Autor:	Sellou H; CNRS, UMR 6290, Institut Génétique et Développement de Rennes, 35043 Rennes, France.; Université de Rennes 1, Structure fédérative de recherche Biosit, 35043 Rennes, France.; Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany., Lebeaupin T; CNRS, UMR 6290, Institut Génétique et Développement de Rennes, 35043 Rennes, France.; Université de Rennes 1, Structure fédérative de recherche Biosit, 35043 Rennes, France.; Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany., Chapuis C; CNRS, UMR 6290, Institut Génétique et Développement de Rennes, 35043 Rennes, France.; Université de Rennes 1, Structure fédérative de recherche Biosit, 35043 Rennes, France., Smith R; Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany., Hegele A; Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany., Singh HR; Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany., Kozlowski M; Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany., Bultmann S; Department of Biology II, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.; Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Ladurner AG; Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany.; Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universität München, 81377 Munich, Germany.; Munich Cluster for Systems Neurology (SyNergy), Biomedical Center Munich, Ludwig-Maximilians-Universität München, 81377 Munich, Germany., Timinszky G; Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, 82152 Planegg-Martinsried, Germany gyula.timinszky@med.lmu.de sebastien.huet@univ-rennes1.fr., Huet S; CNRS, UMR 6290, Institut Génétique et Développement de Rennes, 35043 Rennes, France gyula.timinszky@med.lmu.de sebastien.huet@univ-rennes1.fr.; Université de Rennes 1, Structure fédérative de recherche Biosit, 35043 Rennes, France.
Jazyk:	angličtina
Zdroj:	Molecular biology of the cell [Mol Biol Cell] 2016 Dec 01; Vol. 27 (24), pp. 3791-3799. Date of Electronic Publication: 2016 Oct 12.
DOI:	10.1091/mbc.E16-05-0269
Abstrakt:	Chromatin relaxation is one of the earliest cellular responses to DNA damage. However, what determines these structural changes, including their ATP requirement, is not well understood. Using live-cell imaging and laser microirradiation to induce DNA lesions, we show that the local chromatin relaxation at DNA damage sites is regulated by PARP1 enzymatic activity. We also report that H1 is mobilized at DNA damage sites, but, since this mobilization is largely independent of poly(ADP-ribosyl)ation, it cannot solely explain the chromatin relaxation. Finally, we demonstrate the involvement of Alc1, a poly(ADP-ribose)- and ATP-dependent remodeler, in the chromatin-relaxation process. Deletion of Alc1 impairs chromatin relaxation after DNA damage, while its overexpression strongly enhances relaxation. Altogether our results identify Alc1 as an important player in the fast kinetics of the NAD + - and ATP-dependent chromatin relaxation upon DNA damage in vivo. (© 2016 Sellou, Lebeaupin, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).)
Databáze:	MEDLINE
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