The Drosophila formin Fhos is a primary mediator of sarcomeric thin-filament array assembly.

Autor:	Shwartz A; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel., Dhanyasi N; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel., Schejter ED; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel., Shilo BZ; Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2016 Oct 12; Vol. 5. Date of Electronic Publication: 2016 Oct 12.
DOI:	10.7554/eLife.16540
Abstrakt:	Actin-based thin filament arrays constitute a fundamental core component of muscle sarcomeres. We have used formation of the Drosophila indirect flight musculature for studying the assembly and maturation of thin-filament arrays in a skeletal muscle model system. Employing GFP-tagged actin monomer incorporation, we identify several distinct phases in the dynamic construction of thin-filament arrays. This sequence includes assembly of nascent arrays after an initial period of intensive microfilament synthesis, followed by array elongation, primarily from filament pointed-ends, radial growth of the arrays via recruitment of peripheral filaments and continuous barbed-end turnover. Using genetic approaches we have identified Fhos, the single Drosophila homolog of the FHOD sub-family of formins, as a primary and versatile mediator of IFM thin-filament organization. Localization of Fhos to the barbed-ends of the arrays, achieved via a novel N-terminal domain, appears to be a critical aspect of its sarcomeric roles. Competing Interests: The authors declare that no competing interests exist.
Databáze:	MEDLINE
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