| Autor: |
Rana S; Chemistry Division, Bhabha Atomic Research Centre, Mumbai - 400085, India. kcbarick@barc.gov.in hassan@barc.gov.in and Homi Bhabha National Institute, Anushaktinagar, Mumbai - 400094, India., Shetake NG; Homi Bhabha National Institute, Anushaktinagar, Mumbai - 400094, India and Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai - 400085, India., Barick KC; Chemistry Division, Bhabha Atomic Research Centre, Mumbai - 400085, India. kcbarick@barc.gov.in hassan@barc.gov.in., Pandey BN; Homi Bhabha National Institute, Anushaktinagar, Mumbai - 400094, India and Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai - 400085, India., Salunke HG; Homi Bhabha National Institute, Anushaktinagar, Mumbai - 400094, India and Technical Physics Division, Bhabha Atomic Research Centre, Mumbai - 400085, India., Hassan PA; Chemistry Division, Bhabha Atomic Research Centre, Mumbai - 400085, India. kcbarick@barc.gov.in hassan@barc.gov.in and Homi Bhabha National Institute, Anushaktinagar, Mumbai - 400094, India. |
| Abstrakt: |
The interfacial engineering of magnetic nanoparticles (MNPs) with specific functional groups or targeting ligands is important for their in vivo applications. We report here the preparation and characterization of bifunctional magnetic nanoparticles (BMNPs) which contain a carboxylic moiety for drug binding and an amine moiety for folate mediated drug targeting. BMNPs were prepared by introducing bioactive cysteine molecules onto the surface of undecenoic acid coated Fe 3 O 4 magnetic nanoparticles (UMNPs) via a thiol-ene click reaction and then, folic acid was conjugated with these BMNPs through an EDC-NHS coupling reaction. X-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis indicate the formation of highly crystalline single-phase Fe 3 O 4 nanostructures. The changes in the interfacial characteristics of the nanoparticles and the presence of an organic coating are evident from Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), zeta-potential measurement, and thermogravimetric analysis (TGA). These nanocarriers have an average size of 10 nm, and have a pH dependent charge conversional feature and protein resistance characteristic in physiological medium. These nanoparticles also show high loading affinity for an anticancer drug, doxorubicin hydrochloride (DOX) and its pH dependent release. This is highly beneficial for cancer therapy as the relatively low pH in tumors will specifically stimulate the drug release at the site of interest. Furthermore, our fluorescence microscopy and flow cytometry studies confirmed the higher cellular internalization capability of these folic acid conjugated nanoparticles in cancer cells over-expressing folate receptors. |
