Studies of G-quadruplexes formed within self-assembled DNA mini-circles.

Autor: Klejevskaja B; Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, London, SW7 2AZ, UK. l.ying@imperial.ac.uk., Pyne AL; London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK., Reynolds M; Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk., Shivalingam A; Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk., Thorogate R; London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK., Hoogenboom BW; London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK and Department of Physics and Astronomy, University College London, London, WC1E 6BT, UK., Ying L; Institute of Chemical Biology, Imperial College London, London, SW7 2AZ, UK. l.ying@imperial.ac.uk and Molecular Medicine, National Heart and Lung Institute, Imperial College London, London, SW7 2AZ, UK., Vilar R; Department of Chemistry, Imperial College London, London, SW7 2AZ, UK. r.vilar@imperial.ac.uk and Institute of Chemical Biology, Imperial College London, London, SW7 2AZ, UK. l.ying@imperial.ac.uk.
Jazyk: angličtina
Zdroj: Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2016 Oct 13; Vol. 52 (84), pp. 12454-12457.
DOI: 10.1039/c6cc07110d
Abstrakt: We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.
Databáze: MEDLINE