Date seed characterisation, substrate extraction and process modelling for the production of polyhydroxybutyrate by Cupriavidus necator.

Autor: Yousuf RG; School of Chemical Engineering and Analytical Science, The Mill, The University of Manchester, Manchester M13 9PL, UK; Chemical Engineering Department, University of Baghdad, Iraq., Winterburn JB; School of Chemical Engineering and Analytical Science, The Mill, The University of Manchester, Manchester M13 9PL, UK. Electronic address: james.winterburn@manchester.ac.uk.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2016 Dec; Vol. 222, pp. 242-251. Date of Electronic Publication: 2016 Sep 29.
DOI: 10.1016/j.biortech.2016.09.107
Abstrakt: Poly-3-hydroxybutrate (PHB) is a biodegradable polymer synthesised via bacterial fermentation as a means of storing carbon and energy under unbalanced growth conditions. The production cost of petroleum-based plastics is currently lower than that for biopolymers, and the carbon source is the most significant contributor to biopolymer production cost. A feasibility study to assess the suitability of using a date seed derived media as an alternative for PHB production under various stress conditions was investigated. Results include fructose extraction from date seeds and a mass transfer model to describe the process, demonstrating that the high nutrient content of date seeds makes them a promising raw material for microbial growth and that a meaningful amount of PHB can be produced without supplementation. Maximum dry cell weight and PHB concentrations were 6.3g/l and 4.6g/l respectively, giving a PHB content of 73%, when an initial fructose concentration of 10.8g/l was used.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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